The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, at least one substance of the group of Functional Inhibitors of Acid Sphingomyelinase (FIASMAs) and at least one polypeptide of the GF- superfamily with the ability to inhibit stem cell differentiation, its use in the culture of human pluripotent stem cells, a cell culture system comprising human pluripotent stem cells and the chemically defined medium, as well as a kit for proliferation and/or maintenance of human pluripotent stem cells.

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

The present invention provides an in vitromethod for obtaining cells of the pancreatic endocrine lineage, comprising a step of culturing pancreatic progenitor cells, wherein said pancreatic progenitor cells are in a cell culture medium comprising at least one BET inhibitor.

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

The present invention relates to a cell culture medium using mammalian cells for producing a target material at high efficiency in the mammalian cells, a cell culturing method using same, and a method of producing the target material and, more particularly, to a cell culture medium including Zn ions at a concentration of 30M or more in a culture, a salt thereof, or a chelate compound, a cell culturing method using same, and a method of producing a target material. According to the present invention, a cell culture medium for producing recombinant protein by using mammalian cells can be provided, which can achieve excellent effects in increasing antibody production, without showing any cell growth inhibiting effects.

Disclosed herein are methods for generating SC- cells using chemically defined, completely serum free media, and isolated populations of SC- cells for use in various applications, such as cell therapy.

The present invention relates to the field of fermentation biotechnology, more particularly to methods for the fermentative production of massoia lactone by Aureobasidium species.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

The invention relates to methods of differentiation, isolation, propagation, and storage of small colony variants (SCVs) of E. coli, preferably E. coli 83972 or E. coli HU2117, or modified or variant forms thereof, and methods for using the prepared SCV bacteria to establish probiotic biofilms in treated subjects and/or on treated medical devices.

The present invention relates to a method for providing a fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism, the method comprises the step of: (i) providing a main growth solution; (ii) optionally sterilizing the main growth solution provided in step (i); (iii) providing a trace metal solution; and (iv) mixing the main growth solution (as provided in step (i) or (ii)) with the trace metal solution provided in step (iii) and providing the fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism.