A recombinant vaccine comprising a serotype 9 fowl adenovirus vector (FAdV-9) having at least one exogenous nucleotide sequence inserted encoding at least one antigen of a disease of interest and replacing the adenovirus genome non-essential region, and a pharmaceutically acceptable vehicle, adjuvant and/or excipient, wherein the at least one exogenous nucleotide sequence encoding at least one antigen of a disease of interest and replacing the adenovirus genome non-essential region is located between the 491 and 2782 nucleotides. The vector of this vaccine is stable for industrial scale production. When administering this vaccine in combination with a vaccine against Marek's disease, both vaccines produce an adequate immune response which is not affected by interference between each other. In the same way, effectiveness of the recombinant vaccine is not affected by maternal antibodies, and is capable of inducing both an early and lasting protective response, even with only one application.

Disclosed is a vaccine comprising fiber (2) protein of Fowl Adeno-virus C (FAdV-C) or an immunogenic fragment thereof for use in preventing hepatitis-hydropericardium Syndrome (HHS) in birds, preferably in poultry, especially in broilers.

The invention provides compositions and formulations and use of these compositions and formulations to preserve viral vectors. In particular, the particular embodiments relate to compositions and formulations of trehalose or derivatives thereof as suitable protectants for long-term storage of viral vectors and for both in vitro and in vivo applications

The present invention provides a fowl adenovirus (FAdV) subunit vaccine, comprising at least a chimeric FAdV fiber protein and an adjuvant. This vaccine may be used to ameliorate or prevent adenoviral gizzard erosion (AGE), inclusion body hepatitis (IBH) or hepatitis-hydropericardium syndrome (HHS) in birds. The invention further relates to a method of producing an FAdV subunit vaccine, comprising the steps of expressing a chimeric FAdV fiber protein in an expression system, purifying the fiber protein, and combining the fiber protein with an adjuvant to obtain the FAdV subunit vaccine.