This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

In various embodiments methods of utilizing gene drive constructs in asexual organisms such as viruses are provided. In certain embodiments the methods involve transfecting or infecting a cell with a modified DNA virus containing a gene drive construct; and infecting the cell with the target virus where the genome of said target DNA virus is modified by insertion of the gene drive construct into the genome of the target DNA virus and a population of modified target viruses (containing the gene drive construct) is produced.

In various embodiments methods of utilizing gene drive constructs in asexual organisms such as viruses are provided. In certain embodiments the methods involve transfecting or infecting a cell with a modified DNA virus containing a gene drive construct; and infecting the cell with the target virus where the genome of said target DNA virus is modified by insertion of the gene drive construct into the genome of the target DNA virus and a population of modified target viruses (containing the gene drive construct) is produced.

A tool virus derived from genetically engineered MCMV K181 strain for tracing MCMV infection, wherein the tool virus is a recombinant MCMV K181 strain comprising a tracing elements expression cassette and a BAC backbone, wherein the BAC backbone and the tracing elements expression cassette are inserted together between M06 open reading frame and M07 open reading frame of K181 strain genome; wherein the tracing elements expression cassette comprises three tracing elements coding sequences; and wherein the tracing elements expression cassette from 5 to 3 sequentially comprises a first fluorescent protein, a first linker, a second fluorescent protein, a second linker, a luciferase and a polyA.