There is an infectious full-length clone of Zika virus or a variant thereof, and a use thereof. A full-length clone of Zika virus or a derivative thereof containing a T7 bacteriophage promoter can be used to analyze the replication mechanism, life cycle, and pathogenicity of Zika virus. The clone can be used for screening a drug for preventing or treating Zika virus infections and for evaluating the efficacy of diagnostic techniques. Genetic materials, proteins, or fragments of Zika virus restored from the clone or a derivative thereof can be used as vaccines for preventing Zika virus infections.