Methods and compositions are provided for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas axonopodis in a plant. The invention provides novel bacteriophages virulent to Xylella fastidiosa or Xanthomonas axonopodis, including XfaMija and XfaMijo, and further provides methods for treating or preventing Pierce's Disease or Citrus Canker in plants.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and/or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Methods and compositions are provided for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas axonopodis in a plant. The invention provides novel bacteriophages virulent to Xylella fastidiosa or Xanthomonas axonopodis, including XfaMija and XfaMijo, and further provides methods for treating or preventing Pierce's Disease or Citrus Canker in plants.

Host-specific bacteriophages that kill or prevent growth of shigatoxigenic Escherichia coli (STEC) are provided. The bacteriophages are specific for STEC, exhibit high lytic activity, pH, and thermal stability, and are used as bio-control agents in the meat and produce industry. Notably, the bacteriophages prevent or reduce STEC biofilm formation.

The present invention relates to Siphoviridae bacteriophage Vib-ANP-1 (accession number KCTC 13075BP) having the ability to specifically kill Vibrio anguillarum bacteria and a genome represented by SEQ ID NO: 1 and isolated from nature, and a method for prevention or treatment of Vibrio anguillarum bacterial infection by using a composition containing the same bacteriophage as an effective ingredient.

Methods and compositions are provided for preventing or reducing symptoms or disease associated with Xylella fastidiosa or Xanthomonas axonopodis in a plant. The invention provides novel bacteriophages virulent to Xylella fastidiosa or Xanthomonas axonopodis, including XfaMija and XfaMijo, and further provides methods for treating or preventing Pierce's Disease or Citrus Canker in plants.

The present invention relates to a Siphoviridae bacteriophage Lac-BRP-1 that is isolated from the nature and can kill Lactobacillus brevis cells specifically, which has a genome represented by the nucleotide sequence of SEQ. ID. NO: 1 (Accession NO: KCTC 12659BP), and a method for preventing and treating the contaminations of Lactobacillus brevis by using the composition comprising the bacteriophage as an active ingredient.

The present invention relates to a Siphoviridae bacteriophage Lac-PLP-1 that is isolated from the nature and can kill Lactobacillus plantarum cells specifically, which has a genome represented by the nucleotide sequence of SEQ. ID. NO: 1 (Accession NO: KCTC 12665BP), and a method for preventing and treating the contaminations of Lactobacillus plantarum by using the composition comprising the bacteriophage as an active ingredient.

The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.

Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is Listeria.