Provided is a method which allows, for example, suppression of foaming in the purification step such as distillation and continuous operation, as well as direct treatment of a waste liquid (can liquid) without having to subject the same to an extra purification treatment by removing the microorganisms, nitrogen compounds, and phosphorous compounds at once from an organic substance-containing liquid obtained from microbial fermentation. Also disclosed is a method for producing an organic substance, comprising a microbial fermentation step, a separation step, a liquefaction step, and a second purification step, wherein the concentration of the nitrogen compound in the second can liquid is 0 to 150 ppm based on the total mass of the second can liquid, and the concentration of the phosphorous compound in the second can liquid is 0 to 5 ppm based on the total mass of the second can liquid.

Provided is a method which allows, for example, suppression of foaming in the purification step such as distillation and continuous operation, as well as direct treatment of a waste liquid (can liquid) without having to subject the same to an extra purification treatment by removing the microorganisms, nitrogen compounds, and phosphorous compounds at once from an organic substance-containing liquid obtained from microbial fermentation. Also disclosed is a method for producing an organic substance, comprising a microbial fermentation step, a separation step, a liquefaction step, and a second purification step, wherein the concentration of the nitrogen compound in the second can liquid is 0 to 150 ppm based on the total mass of the second can liquid, and the concentration of the phosphorous compound in the second can liquid is 0 to 5 ppm based on the total mass of the second can liquid.

A process for producing ethanol from lactose containing substrates, comprising simultaneously saccharifying the substrate to produce monosaccharide and fermenting the monosaccharide to produce ethanol at a pH from 3.5-5.5, using a fermenting organism, wherein saccharification is carried out in the presence of a lactase, and wherein the fermenting organism is a Saccharomyces sp., and the ratio between the incubation time required for obtaining at least 90% hydrolysis of the lactose present in the substrate (t-.sub.1) and the total fermentation time (t.sub.2) is in the range of 0.1 to 1, and the Saccharomyces sp. is added in amounts that will result in an ethanol yield of at least 70% w/w of the theoretical ethanol yield from lactose by the end of fermentation.

A process for producing ethanol from lactose containing substrates, comprising simultaneously saccharifying the substrate to produce monosaccharide and fermenting the monosaccharide to produce ethanol at a pH from 3.5-5.5, using a fermenting organism, wherein saccharification is carried out in the presence of a lactase, and wherein the fermenting organism is a Saccharomyces sp., and the ratio between the incubation time required for obtaining at least 90% hydrolysis of the lactose present in the substrate (t-.sub.1) and the total fermentation time (t.sub.2) is in the range of 0.1 to 1, and the Saccharomyces sp. is added in amounts that will result in an ethanol yield of at least 70% w/w of the theoretical ethanol yield from lactose by the end of fermentation.

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO.sub.2 in one or more steps of the process.

Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO.sub.2 in one or more steps of the process.

The production method for an organic substance comprises: a step of feeding waste (G0) to a dryer (13); a step of drying the waste (G0) by the dryer (13); a step of feeding the waste (G0) dried by the dryer (13) to a gasifier (14); a step of gasifying the waste (G0) by the gasifier (14) to generate synthetic gas (G1); and a step of bringing the synthetic gas (G1) into contact with a microbial catalyst to generate an organic substance.

The production method for an organic substance comprises: a step of feeding waste (G0) to a dryer (13); a step of drying the waste (G0) by the dryer (13); a step of feeding the waste (G0) dried by the dryer (13) to a gasifier (14); a step of gasifying the waste (G0) by the gasifier (14) to generate synthetic gas (G1); and a step of bringing the synthetic gas (G1) into contact with a microbial catalyst to generate an organic substance.

Provided is a method which allows, for example, suppression of foaming in the purification step such as distillation and continuous operation, as well as direct treatment of a waste liquid (can liquid) without having to subject the same to an extra purification treatment by removing the microorganisms, nitrogen compounds, and phosphorous compounds at once from an organic substance-containing liquid obtained from microbial fermentation. Also disclosed is a method for producing an organic substance, comprising a microbial fermentation step, a separation step, a liquefaction step, and a second purification step, wherein the concentration of the nitrogen compound in the second can liquid is 0 to 150 ppm based on the total mass of the second can liquid, and the concentration of the phosphorous compound in the second can liquid is 0 to 5 ppm based on the total mass of the second can liquid.

Provided is a method which allows, for example, suppression of foaming in the purification step such as distillation and continuous operation, as well as direct treatment of a waste liquid (can liquid) without having to subject the same to an extra purification treatment by removing the microorganisms, nitrogen compounds, and phosphorous compounds at once from an organic substance-containing liquid obtained from microbial fermentation. Also disclosed is a method for producing an organic substance, comprising a microbial fermentation step, a separation step, a liquefaction step, and a second purification step, wherein the concentration of the nitrogen compound in the second can liquid is 0 to 150 ppm based on the total mass of the second can liquid, and the concentration of the phosphorous compound in the second can liquid is 0 to 5 ppm based on the total mass of the second can liquid.