The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.

The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.

The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product;
wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product;
wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

The present disclosure relates to the identification and the use of activators of a mammalian glycerol-3-phosphate phosphatase (hG3PP) for increasing Gro3P conversion to glycerol and glycerol release from a mammalian cell. The activators of hG3PP can be used in the prevention, treatment and/or alleviation of symptoms associated with obesity, type 2 diabetes and/or metabolic syndrome X. The activators of hG3PP can be used in the prevention, treatment and/or alleviation of symptoms associated with cancer.

The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.

The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.