The present invention concerns an enzymatic process for the preparation of an ingredient comprising 1,3-Olein-2-palmitin (OPO), the most abundant triglyceride present in human breast milk. This is achieved by using immobilized lipase from Ther-momyces lanuginosis for producing 1,3-olein-2-palmitin (1,3-Dioleate-2-palmitate-glycerol) using as substrate tripalmitin or triglycerides enriched in palmitic acid at SN-2 position by first alcoholysis in presence of C3 to C5 alcohol (butanol, pentanol, isopropanol) to produce 2-monopalmitin which is purified by selective crystallization at decreased temperature, followed by esterification using the same lipase and oleic acid.

The present invention concerns an enzymatic process for the preparation of a triglyceride ingredient comprising triglycerides present in human breast milk.

The present invention concerns an enzymatic process for the preparation of a triglyceride ingredient comprising triglycerides present in human breast milk.

The present invention relates to a method for increasing the SUS content in an oil or in an olein fraction, comprising performing 1,3-selective enzymatic intraesterification on a natural starting oil or olein fraction prepared therefrom wherein the ratio between SUS and SUU is at least 1:1.5 and the SSS content is low, in particular close to 0%. Further, the present invention relates to 1,3-Selective intraesterified oil or olein, obtainable by performing the present method.

A method for preparing functional edible oil rich in phytosterol esters and diglycerides includes steps of: 1) adding a raw material: adding phytosterol, triglyceride and a molecular sieve into a reactor, wherein a ratio of the phytosterol and the triglyceride is 1:2-1:4, a molecular sieve amount is 50 g/L; heating to 50-60° C. and stirring for 30-60 min, for obtaining a pre-mixture; 2) providing non-aqueous enzymatic transesterification: adding 5-20 g/L lipase into the pre-mixture, adding 100-200 ppm antioxidant, stirring and reacting for 8-12 h with a temperature of 50-60° C. and an atmospheric pressure, stopping heating and naturally cooling to a room temperature; and 3) post-treating: after reaction, removing the lipase and the molecular sieve by centrifugation, for obtaining the functional edible oil. The functional edible oil rich in two nutritional active components is obtained by the one-step method. Products of the present invention do not need separation and purification, and operation is simple.

Disclosed is a method for synthesizing diglyceride using a bubble column reactor. The method comprises the steps of: an immobilized enzyme is placed on the bearing mechanism of the bubble column reactor; a hot bath mechanism is actuated to heat the reactor body to 55-75° C.; glycerol, fatty acid and water are added into a feed chute, preheated to 55-75° C., and then transferred into the reactor body to initiate the reaction; a bubbling mechanism is actuated so that the inert gas is continuously blown into the reactor body via a sieve plate, forming boiling-like bubbles which promotes the mixing and hence to facilitate the reaction; after the reaction, the water bath mechanism and the bubbling mechanism are turned off, the heating and the inert gas circulation are stopped, a compacting mechanism is actuated, and the reaction mixture is settled and layered, thus obtaining an upper layer which is the crude glyceride layer, and a lower layer which is the glycerol layer; and the crude glyceride layer is subjected to two-stage molecular distillation so as to obtain high purity diglyceride.

An enzymatic deacidification method for partial glyceride lipase and PUFA-rich oil, comprising the following steps: 1) mixing a polyunsaturated fatty acid (PUFA)-rich oil with a non-polar organic solvent and a short-chain monohydric alcohol, adding an immobilized partial glyceride lipase to carry out an esterification reaction, wherein the partial glyceride lipase is a mutant obtained by mutating the Phe at the 278th position of Lipase SMG1 as Asn; 2) recovering the immobilized enzyme, and recovering the organic solvent and the monohydric alcohol so as to obtain a deacidified PUFA-rich oil. The partial glyceride lipase does not catalyze alcoholysis of triglyceride and like side reactions, has high deacidification efficiency, low reaction temperature, prevents high temperature oxidation of PUFAs, and the immobilized enzyme may be recovered and reused repeatedly, and thus the present invention has good application prospects in industry.

The present disclosure relates to methods and systems for refining grain oil compositions using an esterase enzyme component, water, bleaching processes, and combinations thereof, and related compositions produced therefrom having one or more reduced color values. The present disclosure also relates to methods of using said compositions, e.g., as mineral oil replacements.

The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.