Disclosed is a method for synthesizing a diglyceride. The diglyceride is obtained by mixing a fatty acid donor with glycerol, partial glyceride lipase, and monoglyceride lipase by adding water, then subjecting the same to an esterification reaction, with a reaction time of 8 to 24 hours, and further separating and purifying the same. In the present invention, monoglyceride lipase is used to promote the reaction efficiency of partial glyceride lipase in the esterification reaction, so as to increase the synthesis rate of diglyceride. Compared with a single enzyme, the synthesis time is shortened by half or more, and 45.50% or more of diglyceride is obtained after the esterification reaction. Since substantially no triglyceride is generated in products, the content of DAG reaches 98% or more after the same has been purified by means of molecular distillation.

The invention provides enzyme particles comprising an immobilized lipolytic enzyme, a hydrophobic polymer, an organic filter aid, and a water-soluble polyol selected from carbohydrates and sugar alcohols. The particles are suitable for enzymatic interesterification of triglycerides, and subsequent separation of the enzyme and triglycerides by filtration.

An ultrasonic-assisted method for preparing phosphatidylserine, comprising the following steps: adding 100-130 parts of phospholipid into a mixture of 150-200 parts of L-serine, 10-20 parts of anhydrous calcium chloride and 400-500 parts of pure water, adding 20-25 parts of phospholipase D for enzymatic hydrolysis reaction, and applying ultrasound in the enzymatic hydrolysis reaction for treatment. The present invention uses an ultrasonic treatment technology to assist phospholipase D to act on phosphatidylcholine and serine to undergo an enzymatic hydrolysis reaction to prepare phosphatidylserine, and at the same time, the ultrasonic frequency, ultrasonic intensity, ultrasonic power, ultrasonic time, ultrasonic temperature, enzyme activity and other parameters are controlled synergistically, so that the enzymatic hydrolysis conversion rate is 98% or higher.

A fat spread product including: at least 5% by weight of water; and from 10% to 95% by weight of a fat composition, wherein the fat composition included: from 10% to 100% by weight of an intraesterified fat or an interesterified fat blend, including HSHO sunflower oil or a fraction derived from HSHO sunflower oil; at most 85% by weight of at least one liquid oil or liquid oil mixture; and at most 5% by weight of at least one fat soluble additive.

Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

An enzymatic deacidification method for partial glyceride lipase and PUFA-rich oil, comprising the following steps: 1) mixing a polyunsaturated fatty acid (PUFA)-rich oil with a non-polar organic solvent and a short-chain monohydric alcohol, adding an immobilized partial glyceride lipase to carry out an esterification reaction, wherein the partial glyceride lipase is a mutant obtained by mutating the Phe at the 278th position of Lipase SMG1 as Asn; 2) recovering the immobilized enzyme, and recovering the organic solvent and the monohydric alcohol so as to obtain a deacidified PUFA-rich oil. The partial glyceride lipase does not catalyze alcoholysis of triglyceride and like side reactions, has high deacidification efficiency, low reaction temperature, prevents high temperature oxidation of PUFAs, and the immobilized enzyme may be recovered and reused repeatedly, and thus the present invention has good application prospects in industry.

An enzymatic process for producing fatty acid triglycerides using a lipase catalyst system that includes a mixture of a supported lipase catalyst and an additive, such as silica gel. The additive is used without adsorbing any of the acyl group donor or acyl group acceptor reactants onto the additive. Use of the catalyst system decreases production reaction time, decreases the temperatures required for reaction, and allows for a single reaction vessel. The catalyst system can be used to efficiently produce medium chain triglycerides or conjugated linoleic acid triglycerides.

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

A method for preparing a non-hydrogenated, non-palm emulsifier composition, comprises the steps of:providing a fatty acid composition comprising at least 80% by weight free fatty acids, andreacting the fatty acid composition with glycerol, wherein the emulsifier composition comprises: at least 20% by weight monoglycerides; less than 60% by weight of diglycerides; and and from 0-80% by weight triglycerides, wherein the weight % is with respect to the total of monoglycerides, diglycerides and triglycerides, and wherein the fatty acid residues bound to the monoglycerides, diglycerides and triglycerides in the emulsifier composition comprise: from 5% to 80% by weight stearic acid (C18:0); from 10% to 80% by weight oleic acid (C18:1); and from 1% to 10% by weight palmitic acid (C16:0), based on the total weight of C8 to C24 fatty acids.