Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.

Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.

Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.

Oils and fats having a high 2-position palmitic acid content, which are used as a raw material for an oil/fat composition containing an XPX triglyceride having palmitic acid linked to position 2 thereof and X linked to positions 1 and 3 thereof, crystalize at low reaction temperature, likely leading to troubles such as clogging of a reaction vessel (X: an unsaturated fatty acid or a saturated fatty acid having not more than 10 carbon atoms). However, according to the present invention, when such oils and fats are mixed with a particular raw material fatty acid or a lower alcohol ester thereof, the cloud point of the raw material mixture can be set to 39.5 C. or lower, and therefore, crystal deposition does not occur even at a low reaction temperature.

Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.

The present invention relates to a process for producing esters comprising reaction of methyl biodiesel or free fatty acids with a polyhydroxylated alcohol, in the presence of a biocatalyst, which is a fermented solid containing lipases of Rhizomucor miehei produced by culturing the microorganism on agricultural waste by solid state fermentation.

The present invention relates to a process for producing esters comprising reaction of methyl biodiesel or free fatty acids with a polyhydroxylated alcohol, in the presence of a biocatalyst, which is a fermented solid containing lipases of Rhizomucor miehei produced by culturing the microorganism on agricultural waste by solid state fermentation.

The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.

Disclosed is a method for producing algae-derived polyunsaturated fatty acids, comprising the steps of a) contacting dry algae with ethanol; b) removing ethanol to obtain crude oil; c) subjecting the crude oil to a transesterification reaction with a lower alcohol to generate fatty acid alkyl esters; d) extracting the fatty acid alkyl esters to result in a phase containing fatty acid alkyl esters; e) collecting the phase containing fatty acid alkyl esters; and f) contacting the collected phase with silica gel. Also disclosed is a method for effectively removing impurities, including chlorophyll, fucoxanthin and beta-carotene, from crude algal fatty acid alkyl esters, comprising contacting the crude algal fatty acid alkyl esters with silica gel in hexane.