The disclosure discloses an enzymatic method for preparation of lecithin polyunsaturated fatty acids (PUFAs), and belongs to the technical field of separation and application of enzyme. A heat treatment procedure is added after a reaction substrate is in contact with an enzyme to adjust the ratio of sn-1 lysophospholipid PUFAs to sn-2 lysophospholipid PUFAs in a reaction product and to promote the production of sn-2 lysophospholipid PUFAs, thereby promoting the production of lecithin PUFAs, which greatly increases the production efficiency of lecithin PUFAs and the lecithin PUFA content in the product. With simple operations and high reaction rate, the method can significantly increase the content of lecithin PUFAs in the product, can effectively avoid the oxidation of PUFA, and has high economic benefits and promising industrial application prospects.

The disclosure discloses an enzymatic method for preparation of lecithin polyunsaturated fatty acids (PUFAs), and belongs to the technical field of separation and application of enzyme. A heat treatment procedure is added after a reaction substrate is in contact with an enzyme to adjust the ratio of sn-1 lysophospholipid PUFAs to sn-2 lysophospholipid PUFAs in a reaction product and to promote the production of sn-2 lysophospholipid PUFAs, thereby promoting the production of lecithin PUFAs, which greatly increases the production efficiency of lecithin PUFAs and the lecithin PUFA content in the product. With simple operations and high reaction rate, the method can significantly increase the content of lecithin PUFAs in the product, can effectively avoid the oxidation of PUFA, and has high economic benefits and promising industrial application prospects.

An enzymatic deacidification method for partial glyceride lipase and PUFA-rich oil, comprising the following steps: 1) mixing a polyunsaturated fatty acid (PUFA)-rich oil with a non-polar organic solvent and a short-chain monohydric alcohol, adding an immobilized partial glyceride lipase to carry out an esterification reaction, wherein the partial glyceride lipase is a mutant obtained by mutating the Phe at the 278th position of Lipase SMG1 as Asn; 2) recovering the immobilized enzyme, and recovering the organic solvent and the monohydric alcohol so as to obtain a deacidified PUFA-rich oil. The partial glyceride lipase does not catalyze alcoholysis of triglyceride and like side reactions, has high deacidification efficiency, low reaction temperature, prevents high temperature oxidation of PUFAs, and the immobilized enzyme may be recovered and reused repeatedly, and thus the present invention has good application prospects in industry.

Provided herein are fermentation methods that improve the nutritional value and physical properties of microbial oil. Specifically, provided is a method of producing oil with increased omega-7 fatty acids. The method comprises culturing oil-producing microorganisms in a fermentation medium with less than 0.3 mg/L zinc, wherein the culturing produces an oil comprising fatty acids, wherein the oil comprises increased omega-7 fatty acids compared to a control oil. Optionally, the oil is isolated from the microorganisms of the culture.

A microbial oil is obtained from Labyrinthulomycetes in which a gene for fatty acid biosynthesis has been disrupted or an expression of the gene has been inhibited to highly accumulate the fatty acid. The microbial oil typically contains: (a) 1.5% or more of arachidonic acid (AA) based on a total amount of fatty acid; (b) 0.2% or more of dihomo-γ-linolenic acid (DGLA) based on the total amount of fatty acid; (c) 0.04% or more of eicosatetraenoic acid (ETA) based on the total amount of fatty acid; (d) 3.8% or more of eicosapentaenoic acid (EPA) based on the total amount of fatty acid; (e) 13.7% or less of n-6 docosapentaenoic acid (n-6DPA) based on the total amount of fatty acid; and (f) 43.9% or less of docosahexaenoic acid (DHA) based on the total amount of fatty acid.

Provided is a method for producing an unsaturated fatty acid oxide (e.g. ω3 fatty acid oxide) that is new and has more beneficial features compared to conventional technologies. This method uses a lipoxygenase-containing composition (e.g. a lipoxygenase-containing composition derived from beans) as a lipoxygenase enzyme, and also uses a freezing method with organic solvent extraction. The present invention makes it possible to produce an unsaturated fatty acid oxide (e.g. ω3 fatty acid oxide) efficiently and at a low cost. The present invention also provides a pharmaceutical composition containing an ω3 fatty acid oxide isolated and purified by this method as an active ingredient.

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

Provided is a method of producing an oil containing polyunsaturated fatty acids by using Schizochytrium sp. The method comprises: performing fermentation using Schizochytrium sp.; resuspending the resultant cells after fermentation in water, adding cellulase and neutral protease for enzymolysis; mixing the enzymatic hydrolyzate with n-hexane, shaking, extracting, centrifuging, and collecting the n-hexane phase; concentrating the n-hexane phase under reduced pressure to remove the n-hexane, and drying to obtain an oil; performing a first crystallization and a second crystallization, and then performing cold filtration to obtain a liquid oil after the second crystallization; and subjecting the liquid oil to deacidification and decolorization. By regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in the Schizochytrium sp. is increased to more than 12%, and the obtained oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid.

The present invention provides an enzymatic method for producing a fatty acid bornyl ester including using borneol and a fatty acid as a substrate for reaction and adding a lipase in a solvent system or a solvent-free system to catalyze the esterification reaction for a period of time to obtain fatty acid bornyl ester. The present method preferably uses fatty acids or their derivatives as acyl donors to prepare fatty acid bornyl esters. By utilizing the characteristics of the substrate, the synthesis process is simple; the reaction efficiency is high; and the content of fatty acid bornyl ester is up to 97%.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.