Disclosed are fluid controllers configured for removable attachment to a fluid introduction device, wherein the fluid controller comprises a body configured to extend at least partially over the fluid introduction device; an orifice arranged relative to the body to facilitate transfer of a fluid from the fluid introduction device through the fluid controller; a fluid retention structure extending outwardly from at least a portion of the body, wherein the fluid retention structure is configured to engage with a wall of a vaginal canal and inhibit a flow of the fluid from the vaginal canal; and at least one connector configured to releasably attach the fluid controller to the fluid introduction device.

Microfluidic systems are configured to process liquid samples that include cumulus-oocyte complexes (COCs), such as raw follicular fluid, in an automated and continuous manner to produce oocytes separate or denuded from surrounding cumulus cells. The systems include a substrate and a channel that can have two or more sub-channels. Each channel includes an inlet, an outlet, and one or more stages arranged in series. Each of the stages includes one or more expansion units and one or more constriction units. At least one stage includes constriction units having jagged internal surfaces, e.g., teeth, that help remove the cumulus cells from the COCs.

A method for enhancing artificial insemination (AI) in mammals, particularly bovine livestock, uses a specialized aqueous mastic solution to substantially increase conception success rates from conventional 60-65% to about 95%. The solution comprises ionic minerals including magnesium chloride, calcium chloride, sodium, potassium, and an emulsion of milled mastic gum (Pistacia lentiscus, preferably Chios Mastiha, particulates 15-40 m) dissolved in water sourced from inland saltwater bodies such as the Great Salt Lake, delivering approximately 72 ionic and trace minerals. The process involves: preparing the uterus by depositing the solution via syringe to maintain pH at 7.35-7.45 and eliminate bacteria; washing donor semen in the solution to isolate healthy, motile sperm and remove debris/reactive elements; and depositing the washed semen directly into the uterus during ovulation, confirmed via luteinizing hormone detection, ultrasound, or chorionic gonadotropin induction.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel. The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel. The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument. Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.