The present invention relates to a novel method for isolating and culturing dermal papilla cells. Particularly, in the isolation and culture method of the present invention, by culturing dermal papilla tissue isolated from the scalp under hypoxic conditions, dermal papilla cells separated from the dermal papilla tissue are rapidly attached to a culture plate, thus having the effect of shortening the period of establishing (harvesting) dermal papilla cells, and the isolated and cultured dermal papilla cells have immunocompatibility, and thus can be effectively used for the allogeneic transplantation of dermal papilla cells.

Provided is a method for regenerating hair follicles by transplanting a composition containing CD36-expressing dermal sheath cells (DSc) to a human. The composition may be prepared by sorting CD36-expressing dermal sheath cells from a hair follicle tissue by use of a cell-sorting technique along with an antibody to CD36. The composition may also contain dermal papilla cells (DPc).

The present invention relates to a method for culturing epidermis-derived stem cells comprising the step of culturing epidermis-derived stem cells in the presence of a three-dimensional extracellular matrix (3D-ECM) and a basal cell culture medium comprising: Epidermal Growth Factor (EGF); and/or a Vascular Endothelial Growth Factor (VEGF); and/or a Fibroblast Growth Factor (FGF); and further a ROCK (Rho-kinase) inhibitor. The present invention further relates to a method for ex vivo de novo generation of epidermis-derived stem cells. Furthermore, the present invention relates to an epidermis-derived stem cell that is obtainable by a method according to the present invention. Uses of said epidermis-derived stem cell, e.g. uses of said epidermis-derived stem cell for in vitro tissue production, in vitro drug discovery screening and medical applications, are also provided herein. The present invention further relates to a cell culture medium that is employed in the context of a method of the present invention.

Pharmaceutical compositions for promoting hair follicle regeneration and their respective preparation methods are provided to overcome the ineffectiveness and low efficiency of the conventional hair follicle regeneration techniques. The methods for preparing the pharmaceutical compositions for promoting hair follicle regeneration include seeding dermal papilla cells and adipocyte lineage cells on a culture material in order for the dermal papilla cells and the adipocyte lineage cells to jointly assemble cell spheres. The pharmaceutical compositions can effectively enhance the effect and efficiency of hair follicle regeneration and thereby promote the regeneration of hair follicles.

The invention relates to the use of germ cells for obtaining a micro hair follicle and to the use thereof for evaluating the effect of cosmetic, pharmaceutical or dermatological products and also for the prophylactic or therapeutic treatment of a state of reduced pilosity.

The invention relates to the use of matrix cells for obtaining a micro hair follicle and to the use thereof for evaluating the effect of cosmetic, pharmaceutical or dermatological products and also for the prophylactic or therapeutic treatment of a state of reduced pilosity.

The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

Provided are compositions of a system and methods of use thereof to transdifferentiate non-hair follicle stem (HFS) cells into non-auditory induced hair follicle stem cells (iHFSCs) and to subsequent use of these cells in the treatment of hair loss.

The present invention relates to a method for culturing hair follicle-derived stem cells and uses thereof, particularly to a method for culturing hair follicle-derived stem cells; hair follicle-derived stem cells prepared by the method; and a pharmaceutical composition for hair loss treatment or hair growth promotion; a quasi-drug composition; and a cosmetic composition, which contains the prepared hair follicle-derived stem cells, a culture of the hair follicle-derived stem cells, or cells differentiated from the hair follicle-derived stem cells as an active ingredient.

According to the culture method of the present invention, stem cells can be effectively isolated and obtained from hair follicle tissue, and the obtained hair follicle-derived stem cells have excellent pluripotency and self-replication ability as well as excellent hair loss treatment and hair growth promotion effects and can be usefully utilized for hair loss improvement, prevention, or treatment.