The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

Disclosed are recombinant endoxylanases that are expressed and exported in high levels in bacteria, in particular Bacillus subtilis. The recombinant endoxylanases are based on an endoxylanase selected from Trichoderma resei or Bacillus pumilus modified with a signal peptide from B. subtilis alpha amylase (AmyE), B. subtilis levanase (SacC), or B. subtilis YwmC. Accordingly, also disclosed are plasmids for transforming bacteria to express and export such recombinant endoxylanases and the resulting recombinant bacterial strains. The disclosure also encompasses compositions for simultaneously degrading and assimilate cellobiose and xylan comprising a recombinant bacteria engineered to produce xylose from hydrolyzing the agricultural biomass and a xylose assimilator and methods of producing value-added products, for example succinate, from agricultural biomass using such compositions.

The present disclosure relates to liquid enzyme formulations containing one or more alpha-amylases for use in starch processing, wherein the pH of the enzyme formulation is about pH 6.0-8.0, and methods of use thereof. The present disclosure further relates to methods of making a liquid enzyme formulation containing one or more alpha-amylase having improved stability, comprising titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.

Lactic acid bacterial cultures, cell populations and articles of manufacture comprising same are disclosed for generating ethanol from lignocellulse.

A filamentous fungus mutant strain in which enzyme production inhibition caused by glucose is suppressed is constructed, and a method of producing a polysaccharide-degrading enzyme, a method of producing a saccharide from biomass, and a method of saccharifying biomass, each using the filamentous fungus, are provided. The filamentous fungus mutant strain in which Sre1 expression is reduced compared to a parent strain or is lost.

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.

The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.

The purpose of the present invention is to provide an agent for improving intestinal flora which can increase the number of beneficial bacteria such as lactic acid bacteria and Bifidobacterium to improve intestinal flora by using an enzyme. A protease consisting of a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 can increase beneficial bacteria such as lactic acid bacteria and Bifidobacterium in intestines to exert an excellent effect of improving intestinal flora.

The present invention relates to xylanase variants, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.

The present disclosure relates to liquid enzyme formulations containing one or more alpha-amylases for use in starch processing, wherein the pH of the enzyme formulation is about pH 6.0-8.0, and methods of use thereof. The present disclosure further relates to methods of making a liquid enzyme formulation containing one or more alpha-amylase having improved stability, comprising titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.