The invention relates to method for the efficient expression and purification and application of a recombinant fusion protein of MANNase and homologues thereof and GLP-1. Through genetic recombination technology, soluble proteins are obtained by using high density fermentation of Pichia pastoris to induce secretory expression, followed by isolation and purification by filtration and concentration to obtain high yield of target proteins. The high fermentation expression and simple isolation steps solve the limitations of the current GLP-1 analogs such as low drug yield, high cost and the need for frequent injections. More importantly, the fusion protein has hypoglycemic effect not only by injection, but also by oral administration for hypoglycemia and weight reduction, which has good application value in obese and pre-diabetic patients. It also provides a basis for further research on the mechanism of hypoglycemia and weight loss of mannanase and homologues thereof with GLP-1 recombinant fusion protein.

A variant of mannanase is disclosed having at least 90% sequence identity with SEQ ID NO: 1 and a substitution of an amino acid in position 256. An enzyme composition, detergent composition, host cell, animal feed, and feed supplement comprising the present variant are disclosed, as well as methods and uses involving the present variant.

This invention relates to the delivery of heterologous peptides or proteins such as therapeutic peptides, therapeutic proteins or antigens to mucosal sites using vesicles derived from the outer membrane of commensal bacteria, recombinant bacteria capable of producing such vesicles, and methods for the production of such vesicles. The invention further relates to an inducible expression system for use in recombinant bacteria.

The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.

The need for a laundry detergent composition which provides improved removal of stains comprising a combination of mannans and other polysaccharides, is met using a combination of detersive surfactant and a cocktail of enzymes comprising a xanthan endoglucanase, a xanthan lyase, and a mannanase.

The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture.

The present description is related to novel mannanases, compositions including mannanase, to methods for producing mannanases and to methods of using mannanases to degrade and modify mannan containing material.

The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Provided are an animal cell strain for use in producing a glycoprotein which uses a high-mannose sugar chain as a main N-glycan structure, a method for use in producing a glycoprotein by using the cell strain, a glycoprotein produced by using the method, and a use thereof. At least two genes from among a Golgi mannosidase and an endoplasmic reticulum mannosidase gene of the cell strain are damaged or knocked out.

The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.