An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) an amino acid substitution on the position corresponding to the position 271, and (b1) at least one other amino acid substitution for at least one of the positions corresponding to the positions 18, 61, 92, 99, 137, 149, 156, 159, 162, 166, 172, 192, 199, 217, 265, or combinations thereof; and/or (b2) an amino acid substitution on the position corresponding to the position 9, and another amino acid substitution for at least one position corresponding to the positions 29, 48, 101, 130, 31, 133, 144, 217, 224, 252, or combinations thereof. Such proteases are useful for in washing or cleaning agents.

An amino acid sequence may have at least 70% sequence identity to the amino acid sequence identified in SEQ ID No. 1 over its entire length, and (a) an amino acid substitution on the position corresponding to the position 271, and (b1) at least one other amino acid substitution for at least one of the positions corresponding to the positions 18, 61, 92, 99, 137, 149, 156, 159, 162, 166, 172, 192, 199, 217, 265, or combinations thereof; and/or (b2) an amino acid substitution on the position corresponding to the position 9, and another amino acid substitution for at least one position corresponding to the positions 29, 48, 101, 130, 31, 133, 144, 217, 224, 252, or combinations thereof. Such proteases are useful for in washing or cleaning agents.

The invention relates to proteases comprising an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and has, in each case based on the numbering according to SEQ ID NO:1, (i) amino acid substitutions, preferably selected from the amino acid substitutions 9T, 144K, 252T and 271E, at the positions corresponding to positions 9, 144, 252 and 271; and (ii) at least one further amino acid substitution at at least one of the positions corresponding to positions 18, 38, 48, 89, 101, 131, 145, 147, 149, 162, 166, 172, 189, 192, 205, 211, 215, 217, 218, 224 and 274. The invention also relates to the production and use of said proteases. Proteases of this type exhibit a very good cleaning performance.

The invention relates to proteases comprising an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and has, in each case based on the numbering according to SEQ ID NO:1, (i) amino acid substitutions, preferably selected from the amino acid substitutions 9T, 144K, 252T and 271E, at the positions corresponding to positions 9, 144, 252 and 271; and (ii) at least one further amino acid substitution at at least one of the positions corresponding to positions 18, 38, 48, 89, 101, 131, 145, 147, 149, 162, 166, 172, 189, 192, 205, 211, 215, 217, 218, 224 and 274. The invention also relates to the production and use of said proteases. Proteases of this type exhibit a very good cleaning performance.

Provided is a mutant protease with improved stability under acidic condition. A mutant protease that consists of the amino acid sequence as shown in SEQ ID NO:2 or an amino acid sequence having an identity of at least 90% thereto, in which the amino acid residue at a position corresponding to position 303 in the amino acid sequence as shown in SEQ ID NO:2 is substituted with another amino acid residue.

Provided is a mutant protease with improved stability under acidic condition. A mutant protease that consists of the amino acid sequence as shown in SEQ ID NO:2 or an amino acid sequence having an identity of at least 90% thereto, in which the amino acid residue at a position corresponding to position 303 in the amino acid sequence as shown in SEQ ID NO:2 is substituted with another amino acid residue.

The present invention relates to protease variants, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising said polynucleotides, host cells expressing said variants, methods of obtaining the variants, detergent compositions comprising said variants, and use of said variants or said detergent compositions.

The present invention has revealed that an antiviral agent containing a serine protease such as subtilisin, nattokinase, and trypsin has an effect of inactivating non-envelope type viruses, and that combining such an antiviral agent containing a serine protease with a cationic polymer enhances the inactivation effect of the antiviral agent against non-envelope type viruses. As a result, an antiviral agent that is highly safe, that has a high virus inactivation ability, and that contains a component that is inexpensive in terms the manufacturing cost and a cationic polymer, was discovered.

The invention relates to detergent compositions comprising a first protease and a second protease having different net charge characteristics as well as novel protease variants.

The invention relates to detergent compositions comprising a first protease and a second protease having different net charge characteristics as well as novel protease variants.