The invention relates to a method for separating a polynucleotide insert from a polynucleotide vector backbone. The backbone has a plurality of cleavage sites distributed such that the backbone is converted into fragments when the sites are cleaved. This allows straightforward separation of the insert from the backbone. The invention also relates to backbones for use in such a method, and to plasmids and kits comprising such backbones.

The present invention is directed to a method for modifying the retention time of RNA on a chromatographic column. The present invention also concerns a method for purifying RNA from a mixture of at least two RNA species. Furthermore, the present invention relates to a method for co-purifying at least two RNA species from a mixture of at least two RNA species. In particular, the present invention provides a method for harmonizing the numbers of A and/or U nucleotides in at least two RNA species. The present invention is also directed to RNA obtainable by said methods, a composition comprising said RNA or a vaccine comprising said RNA and methods for producing such RNA and compositions. Further, the invention concerns a kit, particularly a kit of parts, comprising the RNA, composition or vaccine. The invention is further directed to a method of treating or preventing a disorder or a disease, first and second medical uses of the RNA, composition and vaccine. Moreover, the present invention concerns a method for providing an adapted RNA sequence or an adapted RNA mixture.

The present invention provides new, improved methods for the purification or isolation of a biological molecule of interest, such as plasmid DNA (pDNA) involving an anion exchange (AEX) chromatography step. The method achieves the simple and effective removal of impurities such as RNA, genomic DNA, proteins, cellular fractions, or combinations thereof. The novel methods of the present invention are particularly suitable for large-scale production plants and provide for purified biomolecules (such as pDNA) with excellent quality and good yields, while also allowing for faster processing times and reduced costs.

The present invention refers to a method for isolating a nucleic acid, said method comprising: a) provision of a fluid test sample, which comprises i) a biological sample, ii) a chaotropic agent with a concentration of at least 1 M in the fluid test sample, and iii) a detergent, b) contacting said fluid test sample with a medium for size-exclusion chromatography, and c) purifying the nucleic acid with size- exclusion chromatography. The present invention further relates to the use of any of the methods according to the present invention for detecting a viral infection as well as to a method for detecting a viral infection. The present invention further relates to a kit-of-parts comprising a medium for size- exclusion and a size-exclusion chromatography device for isolating the nucleic acid of the fluid test sample.

The present invention refers to a method for isolating a nucleic acid, said method comprising: a) provision of a fluid test sample, which comprises i) a biological sample, ii) a chaotropic agent with a concentration of at least 1 M in the fluid test sample, and iii) a detergent, b) contacting said fluid test sample with a medium for size-exclusion chromatography, and c) purifying the nucleic acid with size- exclusion chromatography. The present invention further relates to the use of any of the methods according to the present invention for detecting a viral infection as well as to a method for detecting a viral infection. The present invention further relates to a kit-of-parts comprising a medium for size- exclusion and a size-exclusion chromatography device for isolating the nucleic acid of the fluid test sample.

Provided are methods and compositions for negatively and positively selecting for different size nucleic acid (e.g., DNA or RNA) fragments on borosilicate glass fiber membranes, silica and metal oxide surfaces such that only those fragments falling within a desired size range are obtained.

The present disclosure describes a method of treating a sample comprising cells with a process of partial lysing. Cells are exposed to a process such as bead beating that lyses some cells in the mixture. The process generates a resultant sample mixture that is suitable for both cell morphology screening and genetic screening.

The present disclosure relates to compositions, kits and methods that may be used for removal of matrix components, including proteins and lipids, from one or more oligonucleotides.

The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

Systems and methods for identifying DNA strand size and purifying the DNA based on strand size using electrophoresis. The methods include moving, via voltage, a plurality of DNA strands through a separation gel from an inlet of a capillary or passage to either a first outlet or a second outlet dependent on the DNA strand length. In some implementations, the system is a capillary electrophoresis system. In other implementations, the system is a microfluidic lab-on-a-chip.