Provided are high throughput methods for physically linking cDNA molecules derived from mRNA molecules expressed by the same cell, and libraries of linked cDNA molecules produced by the methods. The methods comprise reverse transcribing mRNA from a single cell in a first container to produce cDNA molecules, and linking the cDNA molecules in a second container. The methods unexpectedly produced libraries of cDNA molecules with an increase in the number of molecules that are correctly linked to other molecules derived from the same cell.

This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

The present invention relates to the inhibitor removal and hence, the improvement of isolation of nucleic acids from crude, inhibitor-rich sample materials by separation methods such as magnetic bead technology. Dedicated inhibitor removal solutions for the isolation of nucleic acids from such sample materials comprising mixtures of polyvinylpyrrolidone and ammonium sulfate suitable for said purpose are provided herewith. Furthermore, provided are nucleic acid separation kits containing such inhibitor removal solutions.

A method for nucleic acid isolation comprising: receiving a binding moiety solution within a process chamber; mixing the binding moiety solution with a biological sample, within the process chamber, in order to produce a moiety-sample mixture; incubating the moiety-sample mixture during a time window, thereby producing a solution comprising a set of moiety-bound nucleic acid particles and a waste volume; separating the set of moiety-bound nucleic acid particles from the waste volume; washing the set of moiety-bound nucleic acid particles; and releasing a nucleic acid sample from the set of moiety-bound nucleic acid particles. The method preferably utilizes a binding moiety comprising at least one of poly(allylamine) and polypropylenimine tetramine dendrimer, both of which reversibly bind and unbind to nucleic acids based upon environmental pH.

The present disclosure relates to a single cell analysis system. Disclosed herein is an instrument for single cell processing. The instrument comprises: a motor component; a processing component, which comprises a processing chamber inside and multiple first connecting holes; a container, which comprises a sample collecting reservoir, a waste collecting reservoir, multiple sample loading reservoirs, multiple first microchannels, and a second microchannel; a chip, which is connected under the container and forms a gap with the container, the chip comprises a third microchannel, the bottom of the third microchannel comprises a microwell array; a snap component comprises a feeding beam and a snap body, and a first end of the feeding beam connects to the snap body and a second end of the feeding beam connects to the motor component; and a pneumatic component which connects with the multiple first connecting holes.

Methods and systems for delivering biological materials such as nucleic acids and/or proteins to the interior of a plant cell are provided. Such methods and systems include those where biological material is non-covalently complexed with a magnetic particle and accelerated multiple times toward a plant cell.

The present disclosure relates to compositions, methods and kits for the isolation of extracellular vesicles. The compositions, methods and kits can comprise a high-density liquid reagent that facilitates the sequestration of extracellular vesicles bound to magnetic beads.

Provided are systems and methods of sample preparation and analyte detection.

Provided herein are compositions and methods for extracting cell-free DNA (cfDNA) from a biological sample without the need for pretreatment with proteases prior to binding of the cfDNA to a solid substrate and without a heated elution step. The methods comprise contacting the biological sample with a chelating agent and an oxidizing agent prior to eluting the cell-free DNA from a solid substrate.

A method of enriching nucleic acid, including mixing a sample with a solid phase extraction material; and separating the solid phase extraction material; wherein the solid phase extraction material is glass beads or magnetic beads modified with reduced graphene oxide. Also disclosed is a method of detecting nucleic acid, including mixing a nucleic acid sample enriched by the method above with a probe; and amplifying and detecting an amplification product by electrophoresis.