The present invention is directed to compositions comprising hydrolase-secreting bacteria and fermenting microorganisms and use thereof, such as for fermentative production of ethanol.

The present invention provides for a method to produce a sugar from a biomass, the method comprising: (a) providing a first mixture comprising a solubilized biomass and a distillable acid-base conjugate salt (DABCS) or deep eutectic solvent (DES), wherein (i) the DABCS is a protic ionic liquid (PIL) or a protic salt comprising a DABCS cation and a DABCS anion, and (ii) the DES is any combination of Lewis or Brønsted acid and base comprising any anionic and/or cationic species that have sufficient vapor pressure so that it can be readily distilled; and (b) distilling at least part of the DABCS from the first mixture in order to separate the at least part of the DABCS from the first mixture.

Systems and methods to provide low carbon intensity (CI) transportation fuels through one or more targeted reductions of carbon emissions based upon an analysis of carbon emissions associated with a combination of various options for feedstock procurement, feedstock refining, processing, or transformation, and fuel product distribution pathways to end users. Such options are selected to maintain the total CI (carbon emissions per unit energy) of the transportation fuel below a pre-selected threshold that defines an upper limit of CI for the transportation fuel.

A method of processing an aqueous hemicellulosic stream containing lignin, comprising: (a) contacting an aqueous hemicellulosic stream containing lignin with a C.sub.3-8 alkyl alcohol at elevated temperature and acidic pH; (b) separating the reaction mixture obtained from step (a) into an aqueous phase containing hemicellulose-derived monosaccharide and an organic phase containing C.sub.3-8 alkyl alcohol; (c) concentrating the organic phase obtained from step (b) to remove at least some C.sub.3-8 alkyl alcohol; (d) treating the residue from step (c) with water or an aqueous medium having an alkaline pH; and (e) recovering C.sub.3-8 alkyl alcohol from the product of step (d).

A method of processing an aqueous hemicellulosic stream containing lignin, comprising: (a) contacting an aqueous hemicellulosic stream containing lignin with a C.sub.3-8 alkyl alcohol at elevated temperature and acidic pH; (b) separating the reaction mixture obtained from step (a) into an aqueous phase containing hemicellulose-derived monosaccharide and an organic phase containing C.sub.3-8 alkyl alcohol; (c) concentrating the organic phase obtained from step (b) to remove at least some C.sub.3-8 alkyl alcohol; (d) treating the residue from step (c) with water or an aqueous medium having an alkaline pH; and (e) recovering C.sub.3-8 alkyl alcohol from the product of step (d).

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen.

Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen.

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).