A method for improving intestinal flora increases the number of beneficial bacteria such as lactic acid bacteria and Bifidobacterium to improve intestinal flora by using an enzyme. The method involves providing a protease, which is a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 that can increase beneficial bacteria such as lactic acid bacteria and Bifidobacterium in intestines to exert an excellent effect of improving intestinal flora.

The present invention relates to isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.

A composition including an alginate lyase enzyme and cleaning adjunct. Methods of treating surfaces by contacting the surface with an aqueous wash liquor having the detergent composition therein.

The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method.

Laundry detergent compositions can include an alginate lyase enzyme, nuclease enzyme, and anionic surfactant. Also included are methods of treating fabrics by contacting the fabric with an aqueous wash liquor having the laundry detergent composition which includes an alginate lyase enzyme, nuclease enzyme, and anionic surfactant.

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Bioactive coatings are provided with two component solvent borne resin and an enzyme dispersed therein. The unique coatings provide for enzyme dispersion in the two component solvent borne resin in aggregates with small particle sizes of up to 5 micrometers. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Laundry detergent compositions including alginate lyase enzyme, hexosaminidase enzyme and anionic surfactant. Methods of treating fabrics by contacting the fabric with an aqueous wash liquor having the detergent composition therein. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits, collar and/or cuff cleaning, anti-redeposition benefits and/or for improved drying of a fabric.

Compositions including an alginate lyase enzyme and anionic surfactant. Methods of treating fabrics by contacting the fabric with an aqueous wash liquor having a detergent composition with an alginate lyase enzyme.

Bioactive coatings that are stabilized against inactivation by weathering are provided including a base including an enzyme associated therein, and a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.