A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and β-glucosidase and substantially free of β-xylosidase activity during hydrolysis.

The present invention relates to a new polypeptide with xylanase activity, the nucleotide sequence encoding it, an ingredient containing said polypeptide, and a process for preparing a food product which comprises adding said ingredient to the preparation. This new polypeptide with xylanase activity is particularly useful for the baking industry, endowing doughs with greater elasticity, less tenacity, formation of a stronger gluten network with less water, shorter mixing time, and greater volume of the final product.

A process for improving wheat straw degradation in a ruminant, including the following steps: a) providing the wheat straw, b) chemically pretreating the provided straw, c) feeding the ruminant with the chemically pretreated wheat straw, wherein the process is characterized in that it also includes a step d) of directly administering an enzymatic rumen additive to the ruminant, i.e. the enzymatic rumen additive is not used as a pretreatment on the wheat straw. Also, the direct use of an enzymatic rumen additive in a ruminant before, during or after feeding the ruminant with wheat straw having undergone a chemical pretreatment, i.e. the enzymatic additive is not used as a pretreatment on the wheat straw.

The present invention relates to an improved process of treating crop kernels to provide a starch product of high quality suitable for conversion of starch into mono- and oligosaccharides, ethanol, sweeteners, etc. The present invention also relates to polypeptides having GH10 xylanase activity and polypeptides having GH62 arabinofuranosidase activity. Further, the present invention also relates to a process for extraction or separation of crude palm oil.

A xylanase-containing feed additive for cereal animal feed is described to facilitate degradation of insoluble glucuronoxylan.

The application relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.

The present invention relates to the technical field of protein engineering, and, in particular, to a xylanase mutant with improved heat tolerance. The present invention artificially introduces two or three unnatural disulfide bridges into xylanase by site-directed mutagenesis and obtains the mutants XynA1 and XynA2 with improved heat tolerance, especially the mutant XynA2 into which three disulfide bridges are introduced, achieving residual enzyme activity of 75% after treatment at 80° C. for 5 min, 72% higher than the residual enzyme activity of the mutant XynA1. The present invention further obtains, by screening, three mutation sites Q51N, H143K, and Q161F that can significantly increase the heat tolerance of mutants, and introduction of the mutation sites into the XynA1 and XynA2 xylanases, whether at a single point or a combination of two points or a combination of three points, can effectively improve the heat tolerance of the mutants.

Provided is a method for safely and selectively producing a substrate culture product including a large amount of a desired degrading enzyme. A method for producing a substrate culture product used for feedstuff includes inoculating filamentous fungi bred so that a target degrading enzyme is produced by self-cloning in high productivity on a substrate, and producing the substrate culture product having functionality by ventilating the substrate to carry out solid culture.

The present invention relates to novel polypeptides with xylanase activity, especially xylanase variants, such as genetically engineered xylanase variants, which show improved thermostability, improved resistance against acid treatment and increased enzyme activity on arabinoxylan. The invention includes the use of said polypeptides in applications, such as for food or feed, for brewing or malting, for the treatment of xylan containing raw materials like grain-based materials, e.g. for the production of biofuels or other fermentation products, including biochemicals, and/or for the wheat gluten-starch separation industry, and methods using these polypeptides, as well as compositions (such as feed additive compositions) comprising said polypeptides.

The present invention relates to an enzyme granulate comprising a carrier and an enzyme, and wherein the granulate comprises from 0.015 to 0.4 wt % of vegetable oil, and a process for preparing an enzyme granulate, comprising mixing a carrier with an enzyme and adding a composition comprising a vegetable oil, wherein the enzyme granulate comprises from 0.015 wt % to 0.4 wt % of the vegetable oil. The invention further relates to the use of the enzyme granulate in the preparation of a food or feed product.