Modified Ochrobactrum strains, methods of producing such modified Ochrobactrum strains, and methods of using such modified Ochrobactrum strains for producing transformed plants are disclosed herein.

The invention provides nucleotide sequences and amino acid sequences of the Dip gene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.

The invention relates to a circular vector for plant transformation comprising: a first tethering nucleic acid and a second tethering nucleic acid; a nucleic acid encoding a polynucleotide of interest (POI); a nucleic acid comprising an origin of replication; and two or more nucleic acids encoding replicon proteins and methods for using the same. Also provided are plants produced by the methods of the invention and products produced from the plant.

Methods and compositions are provided which allow for genetic modification of host cells, including plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacteria and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell and comprises a transformation vector comprising a recombinant DNA construct.

The present technology is in the field of molecular biology and plant biology, particularly as it pertains to gene editing in plants of the genus Cannabis. More specifically, there are provided systems and methods for targeted DNA modifications and gene editing in Cannabis plant, including systems and methods for making such modifications using a CRISPR/Cas-system nuclease complex, kits comprising the CRISPR/Cas-system nuclease complex, and constructs encoding the same.

The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS.sub.1-casein, an αS.sub.2-casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS.sub.1-casein, the αS.sub.2-casein, the β-casein.

A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS.sub.1-casein, an αS.sub.2-casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS.sub.1-casein, the αS.sub.2-casein, the β-casein.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to a method of detecting the presence of an antibody to an avian influenza hemagglutinin antigen in a sample from a subject, wherein the antibody binds to an epitope of an avian influenza hemagglutinin antigen, comprising the steps of cloning a nucleic acid encoding an avian influenza antigen into a vector, infiltrating a plant cell with the vector, recovering the antigen from the plant, contacting the sample with the antigen, and detecting the formation of an antibody-antigen complex, wherein the antibody-antigen complex comprises antibodies in the sample bound to the antigen and wherein the formation of an antibody-antigen complex confirms that the subject has been exposed to an avian influenza hemagglutinin antigen. The present invention also relates to a device for assaying the presence of an antibody to avian influenza hemagglutinin antigen in a sample from a subject.