Plant cell cultures as well as related methods, systems, and compositions for increasing the frequency and efficiency of plant genome editing are provided. Various plant cell growth conditions and/or treatments where such increases in gene editing frequencies are obtained are disclosed.

The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology.

Methods of in vitro clonal propagation, regeneration and transformation in Cannabis are provided. Also provided is the use of such methods in improvements of cannabis cultivars such as via breeding.

Method and apparatus for feeding sand molds laterally and/o between stations of differing heights. The method and apparatus of this invention utilize walking-beam-type conveyors having spaced apart fixed outboard rails and a central reciprocating rail. Walking-beam conveyors can be disposed in perpendicular directions with a junction therebetween that allows for a perpendicular change in direction. The method and apparatus of this invention include lift, leveling, and/or lateral transfer mechanisms for lateral placement of the stations relative to the conveyance path.

A composition including: (i) a ds RNA molecule of at least 18 contiguous nucleotides that are essentially identical or essentially complementary to a plant gene or a transcript of said plant gene; and (ii) a transfer agent that conditions a surface of a plant to permeation by the ds RNA molecule into cells of the plant; wherein permeation of the ds RNA molecule into cells of the plant causes a transient reduction in the expression of the gene and wherein the transient reduction in the expression of the gene causes a change in a yield-associated trait of the plant.

The invention provides novel methods for liquid-mediated delivery of pollen grains to enclosed stigmas in recipient female flowers. For example, methods for liquid-mediated pollination are provided. The methods provided include collecting pollen from a donor plant, suspending the collected pollen in a liquid solution, and introducing said solution to an enclosed stigma of a recipient flower bud on a recipient plant, thereby pollinating the flower with the pollen from the donor plant.

This invention provides polynucleotide molecules and methods for regulating genes in plants, e. g., by providing RNA for systemic regulation of genes. Various aspects of the invention provide polynucleotide molecules and methods for regulating endogenous genes and transgenes in a plant cell and polynucleotide molecules.

Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.

The present disclosure pertains to methods for delivering a compound into a cell comprising a cell wall, including passing a cell suspension through a constriction, wherein said constriction deforms the cell comprising a cell wall, thereby causing a perturbation of the cell such that the compound enters the cell, wherein said cell suspension is contacted with the compound.

Methods for highly efficient and fast generation of transformed plants, highly efficient, mild and fast generation methods for introducing a biomolecule into an intact plant cell by a laser-assisted transfection method, and the regeneration of cell lines, whole tissues or organisms thereof.