A soybean cultivar designated 77130123 is disclosed. The invention relates to the seeds of soybean cultivar 77130123, to the plants of soybean cultivar 77130123, to the plant parts of soybean cultivar 77130123, and to methods for producing progeny of soybean cultivar 77130123. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 77130123. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 77130123, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 77130123 with another soybean cultivar.

A soybean cultivar designated 63301112 is disclosed. The invention relates to the seeds of soybean cultivar 63301112, to the plants of soybean cultivar 63301112, to the plant parts of soybean cultivar 63301112, and to methods for producing progeny of soybean cultivar 63301112. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 63301112. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 63301112, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 63301112 with another soybean cultivar.

The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology.

A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.

An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.

A petunia-calibrachoa plant designated SAKPXC019 is disclosed. Embodiments include the seeds of petunia-calibrachoa SAKPXC019, the plants of petunia-calibrachoa SAKPXC019, to plant parts of petunia-calibrachoa SAKPXC019, and methods for producing a petunia-calibrachoa plant produced by crossing petunia-calibrachoa SAKPXC019 with itself or with another petunia-calibrachoa variety. Embodiments include methods for producing a petunia-calibrachoa plant containing in its genetic material one or more genes or transgenes and the transgenic petunia-calibrachoa plants and plant parts produced by those methods. Embodiments also relate to petunia-calibrachoa varieties, breeding varieties, plant parts, and cells derived from petunia-calibrachoa SAKPXC019, methods for producing other petunia-calibrachoa lines or plant parts derived from petunia-calibrachoa SAKPXC019, and the plants, varieties, and their parts derived from use of those methods. Embodiments further include hybrid seeds, plants, and plant parts produced by crossing petunia-calibrachoa SAKPXC019 with another variety.

The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.

The present invention discloses A METHOD TO PRODUCE PROTEIN IN PENICILLIUM AMAGASAKIENSE'S SLEEPING SPORES BY TRANSFORMATION OF SSRNA. The method includes three steps of culture of Penicillium amagasakiense and collection of spores, pretreatment of Penicillium amagasakiense spores, and electroporation of Penicillium amagasakiense spores by using HDEN method. In the present invention, non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single stranded RNA is introduced into the resting spores of Penicillium amagasakiense by employing the HDEN electrotransformation technique to express protein. The method of this invention is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.

Disclosed herein are compositions and methods for effecting alterations at a defined location in the genome of a non-epidermal plant cell. Further disclosed are methods for providing plants having a modified phenotype or a modified genome.

Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo; (b) a DNA digesting agent; and (c) a targeting RNA, wherein the targeting RNA is capped and/or polyadenylated; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.