Patent classifications
C12N15/8222
DNA constructs for obtaining marker-free transgenic plants
The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
Methods of increasing crop yield under abiotic stress
Methods and materials for increasing abiotic stress tolerance in plants are disclosed. For example, nucleic acids encoding abiotic stress tolerance-increasing polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased tolerance to abiotic stress and methods of increasing plant yield under abiotic stress conditions.
Plant regulatory elements and uses thereof
The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
PLANT REGULATORY ELEMENTS AND USES THEREOF
The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
TRANSGENIC PLANTS WITH ENGINEERED REDOX SENSITIVE MODULATION OF PHOTOSYNTHETIC ANTENNA COMPLEX PIGMENTS AND METHODS FOR MAKING THE SAME
Embodiments of the present invention provide for a transgenic plan, methods of making and DNA constructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a/b ratio at high light intensity and a decrease in the Chl a/b ratio at low light intensity versus wild-type plants grown in the same conditions.
Plant technology
Genetic constructs capable of manipulating fructan biosynthesis in photosynthetic cells of a plant, said genetic constructs include a promoter operatively linked to a nucleic acid encoding a bacterial FT enzyme. These constructs can be used in modification of fructan biosynthesis in plants and, more particularly, for manipulating fructan biosynthesis in photosynthetic cells. The constructs can also be used for increasing plant biomass and, more particularly, for enhancing biomass yield and/or yield stability, including shoot and/or root growth in a plant, and for enhancing the productivity of biochemical pathways.
Gene combination and use thereof
Provided are a gene combination used for controlling foreign gene expression in a specific plant tissue, and a method applying the gene combination to cultivate a transgenic plant. The method is used to cultivate, for example, an endosperm zero expression-type transgenic rice, i.e., rice grain endosperm produced by the rice does not contain any transgenic product protein synthesis and accumulation.
PLANT PROMOTER AND 3'UTR FOR TRANSGENE EXPRESSION
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
Plant regulatory sequence
The present disclosure relates to regulatory sequences. In particular, the disclosure relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The disclosure further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.
Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid. Compositions and methods using promoter inverted repeats to modulate male sterility in plants are provided as well as compositions and methods for restoring fertility.