Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

It was found that it is possible to construct a genome-edited plant in which no exogenous gene is incorporated in a genome by performing negative selection using morphological defects and the like as indices in a regenerated plant originated from a plant cell in which a gene that induces regeneration of the plant and a gene of the genome editing enzyme are introduced.

Insecticidal toxins described herein are fused toxin peptides made up of a targeting domain fused to a toxin domain. The targeting peptide generates a specific association with mosquitoes by causing the fused toxin peptide to bind mosquitoes in a way that leads to the insecticidal activity. Transgenic plants described herein are mosquitocidal by expressing an insecticidal toxin protein in nectar that includes a targeting peptide to ensure specificity against mosquitoes. These transgenic plants serve as role models for safety, since they are non-crop plants and specific to one mosquito species.

The invention relates to methods for increasing seed yield, increasing the total content of protein and/or lipid in seeds and reducing glucosinolate levels by reducing the expression or activity of UPL3. The invention also relates to genetically altered plants characterised by the above phenotypes and methods of producing such plants.

The present disclosure provides compositions and methods related to tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

Compositions and methods are provided for large scale manipulation of genomic regions and chromosomal engineering of plant genomes. Portions of chromosomes may be deleted, translocated, duplicated or inverted, which are useful for various applications in plant breeding programs that relate to recombination, crossovers, and genetic gain. Site-specific directed DNA breaks enhance targeted recombination frequencies, crossover efficiency and movement of large chromosomal segments in crop plant cells. CRISPR-Cas systems enable targeted chromosomal engineering of crop plants.

The present invention relates to cultivated plant of the species Solanum lycopersicum comprising a myb12 allele having one or more mutations, said mutations resulting in production of a mutant myb12 protein, fruits of such plants exhibiting a pink appearance and in addition comprising a mutant cuticle deficiency (cd) allele, whereby the fruits become glossy.

The present invention relates to a method of enhancing the activity of a transcription factor in a plant comprising expressing in said plant a chimeric protein comprising a GIF moiety comprising a GIF domain or fragment thereof, and a DNA binding domain (DBD) moiety. The chimeric proteins comprise a GIF moiety comprising a GIF domain or fragment thereof, or a polypeptide with an amino acid sequence having at least 85%, preferably at least 90%, even more preferably at least 95% sequence identity to the amino acid sequence of a GIF domain or fragment thereof; and a DBD moiety comprising the DBD or plant transcription factor whose activity enhancement is intended, or a binding protein which is capable of forming a tertiary complex with the DBD or transcription factor whose activity modification and/or enhancement is intended. The invention also relates to polynucleotides and polypeptides useful in the method of the invention.

The disclosure relates to a series of independent human-induced non-transgenic mutations found at one or more of the Lip1 genes of a plant; plants having these mutations in one or more of their Lip1 genes; and a method of creating and finding similar and/or additional mutations of Lip1 by screening pooled and/or individual plants. The plants disclosed herein exhibit decreased lipase activity without having the inclusion of foreign nucleic acids in their genomes. Additionally, products produced from the plants disclosed herein exhibit increased hydrolytic and oxidative stability and increased shelf life without having the inclusion of foreign nucleic acids in their genomes.

An isolated protein that is highly abundant in the seed of wheat varieties with desirable breadmaking qualities is provided. Associated promoters and promoter active fragments are also provided. Further provided are plants or plant parts with improved or enhanced breadmaking properties, and methods of producing such plants, wherein the abundance of the isolated protein is increased or enhanced. Such plants and/or plant parts may be used in the production of plant products, for example bread and read products.