The present invention pertains to a method of expressing a protein of interest, preferably a heterologous protein, in preferably a plant. In a preferred embodiment said plant is a doubled haploid homozygous transgenic Nicotiana tabacum plant silenced for Ntp303. Furthermore, the invention relates to said plant with or without nucleic acid constructs according to the invention. Propagation, harvest and tissue material of said transgenic Nicotiana tabacum plant is also a part of the invention.

Provided is a method of increasing the effective tiller number of a rice plant, including overexpressing a gene having a nucleic acid sequence as shown in SEQ ID NO: 1 in the rice plant. The gene encodes a protein which interacts with MOC1 in the rice plant.

To provide a gene that controls the deep rooting of a plant, a transgenic plant introduced with the gene, a method for controlling the deep rooting of a plant using the gene, and such, high-resolution linkage analysis was performed for a genetic locus (Dro1 locus) capable of controlling the deep rooting of a plant, which was detected between a shallow-rooted rice cultivar IR64 and a deep-rooted rice cultivar Kinandang Patong in a large-scale segregating population. As a result, it was revealed that the gene region of Dro1 is located in a region of 6.0 kbp sandwiched between Dro1-INDEL09, which is an InDel marker, and Dro1-CAPS05, which is a CAPS marker. Furthermore, it was confirmed that a transgenic plant transformed with the Kinandang Patong-type Dro1 gene shows a significantly high ratio of deep rooting. It was also confirmed that a plant having the Kinandang Patong-type Dro1 gene is resistant to drought.

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for enhancing mechanical stalk strength in plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a CTL1 polypeptide.

The present invention provides methods and compositions for modulating drought tolerance and/or other agronomic traits in plants. This invention relates to compositions and methods for down-regulating the level and/or activity of PUB10 in plants for creation of plants with improved abiotic stress tolerance, preferably improved drought tolerance. Thus, in one aspect, the present invention provides an isolated nucleic acid comprising a polynucleotide sequence for use in a recombinant DNA construct or a suppression DNA construct for modulating PUB10 expression.

The present invention includes an expression cassette containing a polynucleotide encoding a polypeptide; and a host cell, transgenic plant (e.g., a dicot or monocot), transformed seed, and transgenic rootstock containing said expression cassette. Methods for desensitizing a plant to endogenous cytokinin; increasing seed, embryo or cotyledon size or weight; increasing the seed yield of a plant; and/or increasing the size of the root or root meristem or formation of lateral or adventitious roots are provided. In some embodiments, expression of the polypeptide is under control of a seed-preferred, embryo-preferred or root-preferred promoter.

The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean eukaryotic translation initiation factor 5A-2-likegene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

There are provided compositions and methods for transforming plants, preferably monocot, and even more preferably, sugarcane. The transformation methods involve infection of plant tissue with Agrobacterium, and co-cultivation using culture medium comprising high concentrations of gelling agent, with the result of inhibiting the exacerbated growth of the bacteria and increasing the transformation frequencies. The invention includes regenerating transformed plants, and the transformed plants themselves.

This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.

Compositions and method of delivering a molecule to a plant are provided. In an embodiment, an avenic acid transporter is introduced into a plant. The plant may be a non-graminacious or dicotyledonous plant which does not comprise the transporter in the wild type form. The transporter may be modified to increase uptake of avenic acid along with iron chelated by the avenic acid and/or a molecule conjugated with the avenic acid. Further embodiments provide for conjugating the avenic acid with a molecule for uptake and delivery to the plant. In this manner plant health may be improved by uptake of iron where it would otherwise not occur and/or uptake of the conjugated molecule. The molecule may be a molecule that improves health of the plant. Still further embodiments provide for analogs of avenic acid. Embodiments provide for interplanting Avena sativa which natively produces avenic acid with another plant. Additional embodiments provide for time release of avenic acid provided to a plant.