A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

An object of the present invention is to identify a novel gene that effectively increases plant biomass and to provide said gene as well as techniques utilizing the same. This invention provides a nucleic acid encoding a protein, wherein the protein comprises the amino acid sequence of SEQ ID NO:1 and an amino acid sequence having at least 25% identity or at least 75% similarity to the amino acid sequence of SEQ ID NO:7 or 11, and has an activity to increase plant biomass, as well as techniques utilizing the same.

The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, single kernel weight, ear fresh weight, ear area, ear volume, ear length, number of kernels per ear, and yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more MADS-box polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present disclosure relates to a rice PAL1 gene, an encoding protein and use thereof. The rice PAL1 gene has the nucleotide sequence shown in SEQ ID NO. 1, and the rice PAL1 protein has the amino acid sequence shown in SEQ ID NO. 4. Mutation of the gene leads to reduction of rice plant height and panicle length, while decreasing the number of primary branches, secondary branches and grains per panicle. It is found that the PAL1 gene can restore a mutant panicle type to a normal phenotype. The present disclosure provides a PAL1 gene functioning as a regulator of the rice panicle length and an encoding protein thereof. A rice panicle type is an important trait influencing rice yield. Therefore, it will be desired to directionally design a plant type and improve the rice yield by regulating panicle traits of rice with the PAL1 gene.

This disclosure provides tobacco plants containing a PMT RNAi and tobacco plants having a mutation in PMT, and methods of making and using such plants.

Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Polynucleotides and polypeptides useful in the manufacture of a class of chemical compounds known as alkaloids are provided. The polynucleotides and polypeptides may be used to synthesize alkaloids, including reticuline, thebaine and morphine, in vivo and in vitro. The polynucleotides further may be used to examine the presence of the polynucleotides in a cell or a cell extract, and to modulate expression thereof in living cells.

The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Solanaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods as well as resultant plant varieties developed through the use of self-pollination.

Aspects of the present disclosure relate to genetically altered LysM receptors. In particular, the present disclosure relates replacement of part or all of motifs in the LysM1 domain with the corresponding motifs of the LysM1 domain from a donor LysM receptor that can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)). The present disclosure also relates to genetically altering LysM receptors in plants to include a modified LysM1 domain and to genetically altering LysM receptors in plants by replacement of part or all of motifs in the LysM1 domain. The present disclosure further relates to combining LysM1 domain modifications with modifications of LysM2 domains to include a hydrophobic patch or alter the hydrophobic patch, whereby the LysM2 domain modifications can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)).