Non-transgenic and transgenic plants, preferably crop plants, comprising at least one mutation of the KINTEOCHORE NULL2 (KNL2) protein, especially a mutation causing a substitution of an amino acid within the KNL2 protein, preferably within the C-terminal region of the KNL2 protein, which preferably have the biological activity of a haploid inducer. Further are methods of generating plants and haploid and double haploid plants obtainable by crossing non-transgenic and transgenic plants with wildtype plants as well as methods of facilitating cytoplasm exchange.

The disclosure relates to improvements in methods for efficient editing of two or more endogenous soybean genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the soybean genome.

Provided herein are promoters and related compositions and methods of use. Such promoters are useful for expression cassettes for plants, such as soybean. Such expression cassettes are useful, e.g., to drive expression of trait genes in plants, such as soybean. In some aspects, the disclosure provides an expression cassette comprising a promoter, a 5′ UTR, and an intron.

Provided are compositions and methods for reducing, disrupting, or altering ZmGW2 activity in corn plants. Methods and compositions are also provided for producing modifications in the ZmGW2 gene through mutagenesis and/or editing. Modified plant cells and plants having a modification in the ZmGW2 gene are further provided comprising improved characteristics, such as increased yield.

A method for controlling expression of a nuclear-encoded gene in a plant comprising expressing a chloroplast-encoded dsRNA that silences an endogenous nuclear-encoded gene in the plant to produce a transformed plant line expressing the selected trait.

Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

The invention relates to methods of producing a desired phenotype in a plant by manipulation of gene expression within the plant. The method relates to means which inhibit the level of PK220 gene expression or activity, wherein a desired phenotype such as increased water use efficiency relative to a wild type control plant. The invention also relates to nucleic acid sequences and constructs useful such methods and methods of generating and isolating plants having decreased PK220 expression or activity.

Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 529, 475-528, 530-770, 6179-9796, 9798-10421, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 314, 1-313, 315-474, 771-6178, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing abiotic stress tolerance, yield, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.