Compositions comprising a mixture of proteins derived from MaSP, nucleic acids encoding same and method for the preparation of synthetic dragline spider silk are provided. The compositions of the invention comprise a mixture of proteins of differing molecular weight, wherein each protein of said mixture comprises, independently, multiple repeats of a repetitive region of a MaSP (major ampullate spidroin) protein or a functional homolog, variant, derivative or fragment thereof.

Certain donor plasmid vectors such as pFastBac1 and pFastBac Dual lack a cis DNA element upstream of the polh translation start codon (ATG) present in wild type (wt) Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and contain a SV40 pA fragment. When a cis DNA element is inserted upstream of the 50 bp polh promoter and SV40 pA was replaced with a AcMNPV polh pA signal in pFastBac1 and pFastBacDual, certain protein expression levels in High Five cells using the Bac-to-Bac system reached that of the wt AcMNPV.

Certain donor plasmid vectors such as pFastBac1 and pFastBac Dual lack a cis DNA element upstream of the polh translation start codon (ATG) present in wild type (wt) Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and contain a SV40 pA fragment. When a cis DNA element is inserted upstream of the 50 bp polh promoter and SV40 pA was replaced with a AcMNPV polh pA signal in pFastBac1 and pFastBacDual, certain protein expression levels in High Five cells using the Bac-to-Bac system reached that of the wt AcMNPV.

Described herein, are Bovine immunodeficiency virus gag protein (Bgag) recombinant virus like particles (VLPs) including one or more different types of target pathogen proteins. Also described, are compositions including the Bgag VLPs and the methods of making and using the novel Bgag VLP.

Provided are a constitutive promoter CAR-Mut, an expression construct comprising the promoter and a GAA coding nucleotide sequence functionally linked thereto, a recombinant vector and a host cell. Also provided are a composition and method for delivering a GAA coding polynucleotide to a mammalian cell or an individual using the recombinant vector, and for treating a subject with Pompe disease or acid glucosidase deficiency.

A method for increasing or enhancing baculovirus yield and/or recombinant protein expression from a baculovirus is provided, wherein said method includes the step of engineering said host cell to express one or more of a p35, p35-trunc, p35-trunc-tail, and p49 protein. In some embodiments, said baculovirus does not normally encode a p35 protein, and said method includes the step of engineering said host cell to express one or more of a p35, p35-trunc, and p35-trunc-tail protein. In some embodiments, said baculovirus does not normally encode a p49 protein, and said method includes the step of engineering said host cell to express a p49 protein. Also provided are isolated p35-trunc and p35-trunc-tail proteins.

A method for increasing or enhancing baculovirus yield and/or recombinant protein expression from a baculovirus is provided, wherein said method includes the step of engineering said host cell to express one or more of a p35, p35-trunc, p35-trunc-tail, and p49 protein. In some embodiments, said baculovirus does not normally encode a p35 protein, and said method includes the step of engineering said host cell to express one or more of a p35, p35-trunc, and p35-trunc-tail protein. In some embodiments, said baculovirus does not normally encode a p49 protein, and said method includes the step of engineering said host cell to express a p49 protein. Also provided are isolated p35-trunc and p35-trunc-tail proteins.

The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with a reduced number of or without contaminating baculoviral virions.

The present invention provides a protein fragment for fusion expression of ion channel protein and transport protein. The protein fragment is a Bril protein fragment or a T4L protein fragment. Also provided is a method for preparing a fusion protein by inserting a Brit protein fragment or T4L protein fragment into the N-terminal, C-terminal or intramembrane loop region of the ion channel protein and transport protein so as to improve its, in-vitro stability and crystallizability.

The present invention relates to a recombinant baculovirus comprising: (a) a nucleotide sequence encoding a foreign virus envelope protein; (b) a first promoter operatively linked to the envelope-encoding nucleotide sequence; (c) a nucleotide sequence encoding an antigen protein; and (d) a second promoter operatively linked to the antigen-encoding nucleotide sequence; and a vaccine composition using the same. The recombinant baculovirus of the present invention has an excellent efficacy on both humoral and cellular immune responses against a specific antigen (e.g., HPV L1), enabling to function as a more efficient DNA vaccine.