A method for preparing and applying immobilized enzyme nanogels includes oxidized sodium alginate and enzymes are dissolved separately in deionized water, mixed uniformly, and reacted to obtain immobilized enzyme nanogels; the aldehyde groups in oxidized sodium alginate react with the amino groups in the enzymes, forming imine bonds to immobilize the enzymes; forming nano-scale cross-linked polymer-based particles with a three-dimensional network structure, resulting in the immobilized enzyme nanogels; the surface of immobilized collagenase nanogels is modified with CXCR4 antagonist peptides, which can specifically block CXCR4 on the surface of T cells; the Schiff base bonds can selectively break in the acidic microenvironment of tumors, leading to collagen degradation and reduced extracellular matrix density; the modification enhances the chemotaxis and infiltration of T cells into pancreatic cancer tissues and can inhibit the metastasis of pancreatic cancer.

Described herein are anti-microbial and UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing proteins such as, for example, zinc-related protein/oxidase, silicatein, silaffin, and alcohol dehydrogenase. In some instances, the biological devices also include a gene for lipase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to kill microbial species or prevent microbial growth and to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein. Also disclosed are biological devices producing polyactive carbohydrates and carbo sugars, as well as compositions and articles incorporating both extracts from these devices and the anti-microbial and UV-protective extracts.

Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.

Described herein are anti-microbial and UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing proteins such as, for example, zinc-related protein/oxidase, silicatein, silaffin, and alcohol dehydrogenase. In some instances, the biological devices also include a gene for lipase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to kill microbial species or prevent microbial growth and to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein. Also disclosed are biological devices producing polyactive carbohydrates and carbo sugars, as well as compositions and articles incorporating both extracts from these devices and the anti-microbial and UV-protective extracts.

Provided herein are mutants of estrogen receptor alpha ligand binding domain (ER-LBD), and inducible cell death systems that include mutants of estrogen receptor alpha ligand binding domain (ER-LBD). Also provided are methods of for use of the same, such as inducing cell death in a cell.

Provided are improved cysteine proteases for specifically cleaving and inactivating immunoglobulin G. The improved cysteine pro-teases are useful in methods of treating diseases, disorders or conditions characterized by excessive levels of IgG antibodies, including autoimmune disorders and candidates for organ transplantation sensitized with anti-HLA antibodies, as well as for treating candidates for gene therapy with pre-existing neutralizing antibodies against recombinant vectors and re-dosing of subjects previously treated with a gene therapy vector.

The present invention relates to methods of treating diseases in a subject in need thereof, including proliferative diseases such as cancer, especially solid tumors, as well as other diseases involving invasive cells or entities such as viruses, bacteria, fungi, and parasites, using IgG antibodies, including anti-tumor IgG antibodies, and a protease from Streptococcus pyogenes.