The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.

A protein scaffold includes a plurality of EutM subunits and a multi-enzyme cascade. The multi-enzyme cascade includes a first enzyme attached to the first EutM subunit and a second enzyme attached to the second EutM subunit. The scaffold may be formed by a method that generally includes incubating a plurality of EutM subunits under conditions allowing the EutM subunits to self-assemble into a protein scaffold, attaching a first enzyme of a multi-enzyme cascade to a first EutM subunit, and attaching a second enzyme of the multi-enzyme cascade to a second EutM subunit. The scaffold may be self-assembled in vivo or in vitro. Each enzyme may be, independently of any other enzyme, attached to its EutM subunit in vivo or in vitro. Each enzyme may be, independently of any other enzyme, attached to its EutM subunit before or after the scaffold is assembled.

Provided is a method for purifying -thrombin and for quantifying -thrombin and its degradation polypeptides in a liquid proteinatious solution. The method employs a one-step anion exchange chromatography method. The method allows purification and/or quantification of a homogenous post-translationally modified -thrombin. The method can also be used for purification and/or quantification of -thrombin.

The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.

A system for preparing a thrombin serum that can include a containment device, a cage received within the containment device, a cap attachable to the containment device, an inlet port configured to introduce a non-anti-coagulated autologous blood fluid into the containment device, and an outlet port. An activator, such as glass beads, can be present within the containment device.

The present invention provides methods of producing thrombin using coordinated coexpression systems, and particularly inducible coexpression systems, capable of controlled induction of expression of each gene product required for the production of thrombin.

The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.

Provided are methods for lyophilization of an aqueous thrombin solution, thrombin solutions for use in such lyophilization methods, and solid thrombin compositions produced by such methods.

The present invention is directed to methods for the production of thrombin from a source of prothrombin using a given BaSO.sub.4 reagent as an adsorbent of prothrombin as well as methods for evaluating the suitability of a given BaSO.sub.4 reagent for use in preparation of thrombin. In at least one embodiment, the method includes contacting a sample of the given BaSO.sub.4 reagent with a source of prothrombin to obtain BaSO.sub.4-adsorbed prothrombin, and subsequently evaluating the pro-coagulant activity of the BaSO.sub.4-adsorbed prothrombin. In another embodiment, the method includes the step of evaluating relative to the pro-coagulant activity of normal mammalian plasma.

The present invention provides methods of producing thrombin using coordinated coexpression systems, and particularly inducible coexpression systems, capable of controlled induction of expression of each gene product required for the production of thrombin, and the thrombin produced by this method.