The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

The present disclosure provides pepper plants exhibiting resistance to Phytophthora capsici. Such plants may comprise novel introgressed chromosomal regions associated with disease resistance. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The present disclosure provides methods for testing agronomic performance of transgenic traits and genome edits in plants and for accelerated selection of such plants. Methods evaluated include testing constructs for transgenic trait performance (T) using isolines. The methods comprise crossing events into different genetic backgrounds. Methods also comprise combining isoline data with F2:3 bulk data and developing breeding values across different genetic backgrounds for a higher level of confidence in selecting events.

The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.

Polynucleotides and isolated polypeptides, nucleic acid constructs comprising the isolated polynucleotides, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant are disclosed.

The present invention provides a soybean genetic transformation method using PMI as selectable gene, and relates to the technical field of genetic engineering. In the soybean genetic transformation method of the present invention, using the PMI gene as a selectable marker, soybean explants are infected by recombinant Agrobacterium with the PMI gene and a target gene, followed by co-culture; without recovery culture, the co-cultured explants are directly selected by a selective medium supplemented with mannose, where transformed explants with the PMI gene grow normally under selection pressure of mannose, while non-transformed explant growth is inhibited, thereby selecting successfully transformed positive plants; after shoot elongation and transplantation of the positive plants obtained, genetically transformed soybean plants are obtained successfully. Using the soybean genetic transformation method as provided by the present invention, soybeans can be genetically transformed by PMI genes derived from any species, achieving safe soybean genetic transformation.

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes areas selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

The present disclosure provides pepper plants exhibiting resistance to Phytophthora capsici. Such plants may comprise novel introgressed chromosomal regions associated with disease resistance. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.