Disclosed herein are transgenic plants that overexpress a R2R3-MYB transcription factor gene and methods for increasing or upregulating trichome formation in said plants.

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5’ UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3’ UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmPSID2 gene. Some embodiments relate to a promoter or a 5′ UTR from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a GmPSID2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

A method for promoting expression of a foreign gene in a plant includes: constructing a recombinant vector including a full-length soybean gene promoter pRPS28 or an intron-including soybean gene promoter pRPS28-I, and introducing the recombinant vector into the plant; the full-length promoter pRPS28 being represented by SEQ ID NO: 2, and the intron-including promoter being represented by pRPS28-I SEQ ID NO: 3.

The present technology provides trichome specific promoters of cannabinoid biosynthesis enzyme genes from Cannabis, nucleotide sequences of the trichome specific promoters, and uses of the promoters for modulating the production of cannabinoids and other compounds in organisms. The present technology also provides chimeric genes, vectors, and transgenic cells and organisms, including plant cells and plants, comprising the trichome specific promoters. Also provided are methods for expressing nucleic acid sequences in cells and organisms using the trichome specific promoters.

The present technology provides trichome specific promoters of cannabinoid biosynthesis enzyme genes from Cannabis, nucleotide sequences of the trichome specific promoters, and uses of the promoters for modulating the production of cannabinoids and other compounds in organisms. The present technology also provides chimeric genes, vectors, and transgenic cells and organisms, including plant cells and plants, comprising the trichome specific promoters. Also provided are methods for expressing nucleic acid sequences in cells and organisms using the trichome specific promoters.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

The present disclosure relates to compositions and methods related to tissue-specific promoters and their uses in plants, including tobacco and cannabis. The provided trichome-specific promoters enable the expression of heterologous polynucleotides in trichome tissues.

Composition and methods for the modification of the secondary metabolic functions of glandular trichomes in plants, such as tobacco or Cannabis, that control the formation of terpenes that impart specific flavor and aroma characteristics to the plant leaves are provided. Enhanced terpene production and composition are achieved through targeted modification in the biochemical synthesis pathways for menthol and cis-abienol. This application provides novel nucleotide sequences encoding the enzymology for production of these terpenes in tobacco and Cannabis application to their use plants.

The present disclosure relates to compositions and methods related to modification of trichome density and transport of metabolite and their uses in plants, including tobacco and cannabis. The provided transcription factors enable the increase in trichome density in plants.