The present invention clones a gene ZmNLP5 from maize, which plays an important regulatory role in nitrogen assimilation, and the open reading frame of which has a DNA sequence shown as SEQ ID NO:1. The transcription factor protein encoded by the ZmNLP5 gene has an amino acid sequence shown as SEQ ID NO:2. The uses of the maize NLP transcription factor ZmNLP5 mentioned above in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.2, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.2, in improving nitrogen assimilation in maize, and in promoting elongation growth of maize root in deficient nitrogen environment are further provided.

The present disclosure relates to recombinant prenyltransferase enzymes with increased thermostability and activity and the use of these enzymes in compositions and methods for biosynthesis involving prenylation reactions, including compositions and methods for the preparation of cannabinoids.

Gene-edited soybean plants, plant parts, and plant cells that have a reduced isoflavone content compared to the corresponding unaltered soybean plant, plant part, or plant cell and methods of their production are disclosed. For example, introducing one or more mutations in the coding region of Myb29 gene, the MYBCORE binding motif of the IFS2 promoter, the MYBCORE binding motif of the IFS1 promoter, the coding region of the IFS2 gene, the coding region of the IFS1 gene, or a combination thereof is used to produce the gene-edited plant, plant part or plant cell via targeted genome modification.

The present invention is directed to methods and compositions for breeding plants with an altered flavor or odor profile. Particularly, next generation plant breeding technologies are utilized for producing plants with target genes edited to reduce pungency in flavor or odor. The breeding method is capable of producing mustard plants in which the mustard bomb reaction is disarmed and/or plants with a reduced pungency and/or bitterness in flavor and/or odor.

Disclosed herein are methods for the production of Cannabis meristem explants from dry seeds. Also described are methods of transforming and gene editing using the Cannabis meristem explants disclosed herein.

Enzymes and methods are described herein for manufacturing terpenes, including terpenes.

Transgenic host cells, vectors useful for making transgenic host cells, and kits useful for making transgenic host cells are described. Also described are transgenic plants. In some embodiments, transgenic host cells express a 4-hydroxyphenylacetaldehyde synthase (4HPAAS). In some embodiments, transgenic host cells express a tyrosol:UDP-glucose 8-O-glucosyltransferase (T8GT). The transgenic host cells are useful for biosynthesis of one or more of salidroside, icariside D2, tyrosol, and 4-hydroxypenylacetaldehyde.

Methods are provided for genome editing. On example method includes editing a genome sequence of an organism with multiple edits simultaneously without precise knowledge of a phenotypic effect of each individual one of the multiple edits, wherein the multiple edits are selected based on a prediction of an aggregate phenotypic effect of the multiple edits on a phenotypic trait. The method also includes aggregating the multiple edits into multi-dimensional pools, whereby phenotypic effects of contrasting pools of edits are compared to ascertain which of the multiple edits are most likely to be causing large phenotypic effects while eliminating need to evaluate each edit separately. The organism may include one of: maize, soybean, wheat, sorghum, rice, cotton, rapeseed, sunflower, bean, tomato, squash, cucumber, melon, pepper, watermelon, eggplant, okra, pea, chickpea, lentil, peanut, onion, carrot, celery, beet, cauliflower, broccoli, cabbage, Brussels sprout, radish, black-eyed pea, potato, sweet-potato, sugar cane, cassava, and banana.

The invention relates to molecules for controlling plant growth and development. Specifically, the invention relates to molecules comprising a gibberellin activator or a gibberellin inhibitor operably linked to a promoter specific to a lateral organ primordium. The invention also relates to transgenic plants having the transgenic molecules and methods for making such transgenic plants.

The invention is directed to a patchoulol synthase, to a nucleic acid encoding said patchoulol synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing patchoulol and elemol, and preferably also pogostol, and to a method of preparing a patchoulol synthase.