Provided are methods of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO: 387, 1-386, 388-469, 763-3704 and 3705; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO: 602, 470-601, 603-762, 3706-5858, 5860-5910, 5912, 5914-5923, 5925-6046 or 6047. Also provided is an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 387, 1-386, 388-469, 763-3704 and 3705, which can be used to increase the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant.

Provided is a modified Cannabis plant exhibiting reduced volatile organic compounds (VOCs) emission. The modified Cannabis plant includes at least one targeted gene modification conferring reduced expression or silencing of at least one gene involved in a terpene biosynthesis pathway. Further provided are methods and uses concerning the aforementioned modified Cannabis plant.

The present invention relates to a method of modulating the alkaloid content of a plant or a part thereof, the method comprising modifying said plant by modulating the activity or expression of at least one gene encoding a BTB/POZ NPH3 domain-containing protein. The present invention also relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding a BTB/POZ NPH3 domain-containing protein.

The present invention relates to a method of modulating the alkaloid content of a plant or a part thereof, the method comprising modifying said plant by modulating the activity or expression of at least one gene encoding an RNA binding protein. The present invention also relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) precursor in tobacco, the method comprising modulating the activity or expression of at least one gene encoding an RNA binding protein.

A genetically engineered plant that expresses a 6-phosphogluconate dehydratase (EDD) and/or a 2-keto-3-deoxy-6-phosphogluconate aldolase (EDA) is provided. The plant comprises at least one of a first or second modified gene. The first modified gene comprises a first promoter and a nucleic acid sequence encoding the EDD. The first promoter is non-cognate with respect to the nucleic acid sequence encoding the EDD. The first modified gene is configured so transcription of the nucleic acid sequence encoding the EDD is initiated from the first promoter and results in expression of the EDD. The second modified gene comprises a second promoter and a nucleic acid sequence encoding the EDA. The second promoter is non-cognate with respect to the nucleic acid sequence encoding the EDA. The second modified gene is configured so transcription of the nucleic acid sequence encoding the EDA is initiated from the second promoter and results in expression of the EDA.

The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

The present disclosure provides plant transformation vectors, T-DNA insert regions, and transformed plants. The vectors are designed to be a binary vector for use in plant transformations for such as potato. The transformed plants are characterized in that they contain the T-DNA insert region comprising stacked expression cassettes and the corresponding phenotype. The present disclosure also provides methods for identifying genetic material in transformed plants, including in food products made from such plants. The disclosure further relates to the materials and/or means for detecting plant transformation events and methods for detecting presence of plant transformation events.

The present disclosure relates generally to targeted gene editing constructs, including methods of designing a DNA-recognition moiety for modulation of gene expression in plants, DNA-recognition moieties, gene editing constructs, methods for the modulation of gene expression in plants using gene editing constructs, and plants or regenerable plant cells produced therefrom.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that positively regulates alkaloid biosynthesis, such as nicotine biosynthesis. In particular, the present disclosure provides methods for the inhibition of Nicotiana benthamiana auxin response factor 1 (NbTF1) to reduce alkaloid biosynthesis in plants.

Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.