Methods and compositions are provided for increasing the expression of a gene of interest in a plant by co-expressing the gene of interest with a p21 polynucleotide. The gene of interest can be endogenous or heterologous to the plant. Further provided are plants, such as tobacco plants, comprising a heterologous p21 polynucleotide. Co-expression of the p21 polynucleotide with a gene of interest in the plant increases the expression of the gene of interest when compared to a control plant. Accordingly, p21 co-expression can increase the expression of genes of interest encoding proteins such as defense proteins, enzymes, signaling proteins, reporter proteins, antibodies and fragments thereof, growth factors, cell surface receptor molecules, seed storage proteins, and fungicides in a plant.

A protein capable of converting chlorogenic acid into isochlorogenic acid. The protein includes or is an amino acid sequence SEQ ID No. 1, a sequence having at least 80% identity with this sequence or a fragment of this sequence. Also, a process for producing isochlorogenic acid from chlorogenic acid, which includes producing the protein and bringing it into contact with chlorogenic acid.

The present invention relates to a group of glycosyltransferase, and an application thereof. Specifically, provided is using glycosyltransferase GT29-32, GT29-33, GT29-34, GT29-4, GT29-5, GT29-7, GT29-9, GT29-11, GT29-13, GT29-17, GT29-18, GT29-19, GT29-20, GT29-21, GT29-22, GT29-23, GT29-24, GT29-25, GT29-36, GT29-37, GT29-42, GT29-43, GT29-45, GT29-46, PNUGT29-1, PNUGT29-2, PNUGT29-3, PNUGT29-4, PNUGT29-5, PNUGT29-6, PNUGT29-7, PNUGT29-8, PNUGT29-9, PNUGT29-14, and PNUGT29-15, as well as derived polypeptides thereof to catalyze the first glycosyl at position C-20, the first glycosyl at position C-6, and the first glycosyl at position C-3 of a tetracyclic triterpene compound substrate to elongate a carbohydrate chain, thereby obtaining a catalytic reaction of ginsenoside products such as ginsenoside Rg3, ginsenoside Rd, ginseno-side Rb 1, ginsenoside Rb3, saponin DMGG, saponin DMGX, gypenoside LXXV, gypenoside XVII, gypenoside XIII, gypenoside IX, notoginsenoside U, and notoginsenoside R1, notoginsenoside R2, notoginsenoside R3, 3-0-13-(D-xylopyranosyl)-13-(D-glucopyra-nosyl)-PPD, 3-0-13-(D-xylopyranosyl)-13-(D-glucopyranosyl)-CK, 20-O-Glucosylginsenoside Rf, and Ginsenoside F3. Glycosyltrans-ferase in the present invention can further be applied to construction of artificially synthesized ginsenoside, novel ginsenoside, and derivatives thereof.

This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

The present invention discloses the use of a flavonoid glycoside and glycosyltransferase gene thereof in regulating plant resistance to weeds. The present invention provides the use of tricin-5-O-glucopyranoside in any one of the following: regulating plant allelopathy; regulating plant resistance to weeds; inhibiting the growth of weeds, and the use of Os07g0503900 protein or related biological materials thereof in any one of the following: regulating plant allelopathy; regulating plant resistance to weeds; regulating the content of tricin-5-O-glucopyranoside in plants; catalyzing the glycosylation of tricin to generate tricin-5-O-glucopyranoside; act as or preparing glycosylation transferase; inhibiting the growth of weeds. The present invention is of great significance for developing environment-friendly green pesticides, cultivating rice varieties with high allelopathy and regulating the biosynthesis of tricin-5-O-glucopyranoside, and provides theoretical guidance for the control of weeds in paddy fields.

The disclosure provides nucleic acid constructs that include a TPR-domain suppressor of STIMPY (TSS) promoter operably linked to an isopentenyl-transferase 7 (IPT7) coding sequence. The introduction of such a construct into a plant or plant cell generates transgenic plants having increased root mass and greater carbon sequestration capacity. Plants generated using the methods are provided. Such plants can include other desirable traits.

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

The present invention relates to a method for determining the genotype of a Cruciferous vegetable plant for a plant with an increased glucosinolate level, comprising obtaining a sample of nucleic acids from said plant or a portion thereof and detecting in said nucleic acids a polymorphism at the Myb28 locus that is genetically linked to an increased glucosinolate level. The polymorphism may comprises at least one of: a) a single nucleotide polymorphism (SNP) at a position corresponding to nucleotide 83, 136, 226, 563, 610, 830, 995, 1116, 1513, 1577, 1606, 1620, 1825, 1863, 1877 or 2026 of SEQ ID NO: 1, or b) a polymorphism in the number of nucleotides present between nucleotides 323 and 332, between nucleotides 521 and 524, between nucleotides 783 and 786, between nucleotides and 909 and 914, between nucleotides 1365 and 1369, between 1811 and 1821, or between nucleotides 2046 and 2056 of SEQ ID NO: 1, or c) a polymorphism in the number of nucleotides present between nucleotides 836 and 837, between nucleotides 867 and 868, or between nucleotides 943 and 944 of SEQ ID NO: 1.

A novel maize variety designated PH2T0P and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2T0P with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2T0P through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2T0P or a locus conversion of PH2T0P with another maize variety.

A novel maize variety designated X80H130 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X80H130 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X80H130 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X80H130, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X80H130. This invention further relates to methods for producing maize varieties derived from maize variety X80H130.