This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene. Some embodiments relate to a promoter from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3 UTR from a Panicum virgatum (Pavir.J00490) egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays egg cell gene. Some embodiments relate to a promoter from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3 UTR from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NO: 121, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 95 or 96, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.

The present invention relates to the field of biotechnology and plant breeding, and in particular relates to a method using plant cell proliferation regulator gene to induce haploid, and a use thereof in plant breeding. In the present invention, expression profiles of unfertilized egg cells of rice and rice embryos 5 days after fertilization are analyzed, and 2 genes are discovered to have relatively high expression, OsCPRO1 and OsCPRO2. The research shows that these two genes can be utilized to induce the production of haploid descendants. Specifically, a promoter specifically expressed in egg cells controls the gene to convert a plant, obtaining induced haploid plant material of a positive transgenic plant. Furthermore, the induced haploid plant material can be combined with a MiMe system to obtain apomixes material, and thereby generate clone seeds.

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Glycine max egg cell gene. Some embodiments relate to a promoter or a 5 UTR from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3 UTR or a terminator from a Glycine max egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Compositions and methods are provided for high-efficiency large scale manipulation of genomic regions and chromosomal engineering of plant genomes. Enhancement of chromosomal modification includes the provision of one or more linker oligonucleotides complementary to the distinct chromosomal ends following double strand breaks, increasing the temperature effect and/or recurrent cutting by an endonuclease to facilitate higher chromosomal modification including large segment translocations and recombination. Site-specific directed DNA breaks under one or more of the experimental conditions (including CRISPR-Cas systems) disclosed enhance targeted recombination frequencies, crossover efficiency and movement of large chromosomal segments in crop plant cells.

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:130-258 and 536-791, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays KN1 gene. Some embodiments relate to a promoter from a Zea mays KN1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:130-258 and 536-791, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.

A disk clamp for clamping a plurality of disks within a disk drive has a single fastening hole located at its symmetrical center sized to pass the shaft of a screw having a head diameter larger than the fastening hole. The screw fastens the disk clamp to a motor hub supporting the plurality of disks. The disk clamp has a moat around the fastening hole, at a maximum diameter that is smaller than the head diameter of the head on the fastening screw. The moat may be circular, have spike trenches angled toward the fastening hole, or be spiral. The diameter of the spiral moat decreases in a clockwise or counterclockwise direction toward the fastening hole. The midsection of the disk which the screw head covers is biased at a negative angle toward the fastening hole forcing particles generated during assembly toward the fastening hole of the disk clamp.