C12N15/8297

METHODS AND COMPOSITIONS FOR GENERATING DOMINANT SHORT STATURE ALLELES USING GENOME EDITING

The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of specific subtypes of GA20 oxidase genes to introduce an antisense sequence or segment into the gene. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

METHODS AND COMPOSITIONS FOR GENERATING DOMINANT SHORT STATURE ALLELES USING GENOME EDITING

The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of a specific GA20 oxidase gene or locus to produce a genomic deletion or disruption that brings an antisense sequence of the GA20 oxidase gene under the control of a neighboring SAMT gene promoter. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

DELAYED HARVEST OF SHORT STATURE CORN PLANTS

Methods for delayed harvesting of corn fields are provided herein. These methods provide an extended, flexible period of time to harvest corn. The methods allow growers to harvest their corn at the optimal time for drying down or accessing seed, without increasing the risk of losing yield to lodging.

Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield

The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

COMPOSITIONS AND METHODS FOR IMPROVING CROP YIELDS THROUGH TRAIT STACKING

The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, single kernel weight, ear fresh weight, ear area, ear volume, ear length, number of kernels per ear, and yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more MADS-box polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

PROGRAMMABLE EPIGENETIC CONTROL OF GENE EXPRESSION IN PLANTS

Disclosed herein are artificially synthesized nucleic acid constructs to guide an epigenetic modification for at least partially silencing or activating a target gene in an organism such as a plant or seed, and formulations thereof. Also disclosed are methods of applying such nucleic acid constructs to the plant or to the seed. Also disclosed are engineered seeds and plants obtained by the epigenetic modification.

PROGRAMMABLE EPIGENETIC CONTROL OF GENE EXPRESSION IN PLANTS

Disclosed herein are artificially synthesized nucleic acid constructs to guide an epigenetic modification for at least partially silencing or activating a target gene in an organism such as a plant or seed, and formulations thereof. Also disclosed are methods of applying such nucleic acid constructs to the plant or to the seed. Also disclosed are engineered seeds and plants obtained by the epigenetic modification.

METHODS FOR HYBRID CORN SEED PRODUCTION

Methods for improving the efficiency and productivity of hybrid corn seed production are provided herein. Various methods to improve the transfer of pollen from male corn plants to female corn plants, and thus increase yield, are provided herein. Without being limiting, these methods include varying the height of male and female corn plants in a field, as well as varying the number, arrangement, and ratio of male-to-female rows in a field.

Plants comprising a gibberellic acid 2-oxidase gene expression cassette

The invention provides for an expression cassette comprising a gibberellic acid 2-oxidase gene, and turfgrass plants comprising said cassette.

TRANSGENIC CORN EVENT ZM_BCS216090 AND METHODS FOR DETECTION AND USES THEREOF

The invention provides a transgenic corn event ZM_BCS216090, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event ZM_BCS216090. The invention also provides polynucleotides and sequences specific for event ZM_BCS216090 and methods for using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event ZM_BCS216090 and detecting event ZM_BCS216090, or a polynucleotide or DNA sequence specific for event ZM_BCS216090, in a DNA molecule or sample.