The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

The present invention relates to the use of Argonaute systems in plants for genome engineering, and compositions used in such methods.

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits of interest such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Goss' Wilt, a bacterial disease associated with Clavibacter michiganense spp.

A method for the transformation of sugar beet protoplasts includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. The protoplasts are transformed with a nucleic acid construct including a nucleotide sequence of interest and a selection marker sequence. One or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts are applied to an in vitro culture of the protoplasts. Sugar beet plants are regenerated from the surviving protoplasts having integrated the nucleic acid construct including the sequence of interest and the selection marker sequence. The selection marker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

Disclosed herein are engineered antimicrobial peptides (e.g, HTH peptide or AAPs) and methods of using such peptides to treat pathogenic infections, such as HLB disease and X. fastidiosa, in plants, such as citrus plants and grape plants. The engineered antimicrobial peptides may be derived from amphipathic helical peptides. The engineered antimicrobial peptides disclosed herein may be formed by coupling two or more amphipathic helical peptides. An engineered antimicrobial peptide may include a first amphipathic helical peptide coupled with a second amphipathic helical peptide by a linker domain forming a helix-turn-helix scaffold formation. Such amphipathic helical peptides may be endogenous to a target host, such as a plant (e.g., a citrus plant or grape plant).

The present invention provides an inbred corn line designated JII6578, methods for producing a corn plant by crossing plants of the inbred line JII6578 with plants of another corn plant. The invention further encompasses all parts of inbred corn line JII6578, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line JII6578, and plants produced according to these methods.

The present disclosure relates, in some embodiments, to a method of reducing levels of Candidatus Liberibacter solanacearum (Lso) in a potato, the method including (a) transforming the potato with an expression vector to generate a transformed potato, where the expression vector may include in a 5′ to 3′ direction: an expression control sequence; an exogenous nucleic acid operably linked to the expression control sequence; and a 3′ termination sequence operably linked to the exogenous nucleic acid; and (b) cultivating the transformed potato in conditions suitable for expression of the exogenous nucleic acid. According to some embodiments an exogenous nucleic acid may include a nucleic acid sequence having at least 98% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 6.

The present invention is in the field of soybean variety BY1213086 breeding and development. The present invention particularly relates to the soybean variety BY1213086 and its seed, cells, germplasm, plant parts, and progeny, and methods of using BY1213086 in a breeding program.