A device having: an article having a flat surface and a lower surface opposed to the flat surface; a cavity formed in the lower surface forming a complete loop surrounding a central portion of the article; a heating element having the same shape as the complete loop in the cavity and positioned to warm a portion of the flat surface adjacent to the heating element when the heating element is activated; a cooling device positioned to cool a portion of the flat surface in the central portion; and a release layer on the flat surface. A device having: an article having an upper surface; a heating element on the upper surface forming a complete loop surrounding a central portion of the article; and an electrically insulating material on the upper surface within the central portion.

A charged multilayer nanofiber filter for air filtration and the fabrication method thereof is disclosed. The charged multilayer nanofiber filter has a plurality of charged nanofiber mats. Each of the plurality of charged nanofiber mats is obtained by charging nanofiber mats individually before assembling. The nanofiber mat comprises a nanofiber layer and a substrate layer. The plurality of charged nanofiber mats is assembled such that the nanofiber layers and the substrate layers are stacked together in an alternative manner. The charged multilayer nanofiber filter can be fabricated by electrospinning a polymer solution on the substrate layer to obtain a nanofiber mat, charging the nanofiber mat to obtain a charged nanofiber mat, and assembling a plurality of charged nanofiber mats to form the charged multilayer nanofiber filter.

A continuous wire drive system for a needleless electrospinning apparatus, the electrospinning apparatus including an electrospinning enclosure and within which a nanoscale or submicron scale polymer fiber web is formed onto a substrate from a liquid polymer layer coated onto a plurality of continuous electrode wires passing through the electrospinning enclosure. The continuous wire drive system includes a master wire drive drum and a slave wire drive drum, each of the master wire drive drum and slave wire drive drum including a plurality of wire guides, each of the wire guides including a channel or groove for receiving one of the plurality of continuous electrode wires. The continuous wire drive system is external to the electrospinning apparatus, and the continuous wire drive system drives the plurality of continuous electrode wires through the electrospinning enclosure.

A three-dimensional electrospun biomedical patch includes a first polymeric scaffold having a first structure of deposited electrospun fibers extending in a plurality of directions in three dimensions to facilitate cellular migration for a first period of time upon application of the biomedical patch to a tissue, wherein the first period of time is less than twelve months, and a second polymeric scaffold having a second structure of deposited electrospun fibers. The second structure of deposited electrospun fibers includes the plurality of deposited electrospun fibers configured to provide structural reinforcement for a second period of time upon application of the three-dimensional electrospun biomedical patch to the tissue wherein the second period of time is less than twelve months. The three-dimensional electrospun biomedical patch is sufficiently pliable and resistant to tearing to enable movement of the three-dimensional electrospun biomedical patch with the tissue.

A structure of aligned (e.g., radially and/or polygonally aligned) fibers, and systems and methods for producing and using the same. One or more structures provided may be created using an apparatus that includes one or more first electrodes that define an area and/or partially circumscribe an area. For example, a single first electrode may enclose the area, or a plurality of first electrode(s) may be positioned on at least a portion of the perimeter of the area. A second electrode is positioned within the area. Electrodes with rounded (e.g., convex) surfaces may be arranged in an array, and a fibrous structure created using such electrodes may include an array of wells at positions corresponding to the positions of the electrodes.

Provided herein is a mandrel for use in electrospinning prosthetic valve devices. Also provided are prosthetic valve devices for implantation in an animal or a human. Methods of making and using the valve devices are also provided herein.

The invention is directed to products and methods for preparing self-seeding vascular constructs generated as a bi-layered electrospun matrices, conjugated with EPC-specific antibodies and anti-thrombogenic agents on the inner surfaces of their lumens.

In one embodiment, a fiber manufacturing apparatus has a discharge head which discharges a raw material liquid in which a polymer is dissolved in a solvent toward a collector, and a power source which generates a potential difference between the discharge head and the collector. The fiber manufacturing apparatus further has a recovery device, a cleaning device, and a moving device. The recovery device recovers the raw material liquid to be discharged by the discharge head. The cleaning device cleans the discharge head. The moving device moves the discharge head to any position out of a spinning position where the discharge head and the collector are opposite to each other, a recovery position where the discharge head and the recovery device are opposite to each other, and a cleaning position where the discharge head and the cleaning device are opposite to each other.

A method of forming prefabricated units used in production of systems of prosthetic aortic valve transcatheter implantation and prosthetic aortic valve prefabricated unit with an non-thrombogenic smooth surface layer or with a porous fibrous layer constituting a scaffold for epithelium cell culture, intended for manufacturing TAVI system. Stents for covering and solutions of polycarbonate silicones and/or polycarbonate urethanes and/or polyurethane with average molecular weight in the range from 50 000 g/mol to 200 000 g/mol in the solvent DMAC are prepared. Initially a smooth layer of polycarbonate silicone is applied in the electrospinning machine by electrospraying with use of the solution in DMAC with the concentration of 2-8% w/w. and/or a fiber of polycarbonate urethane is applied by electrospinning on the roller with use of the solution in DMAC with the concentration of 8- 20% w/w to obtain the first surface layer, with a specified speed, number of heads, thickness of capillaries, speed of movement, voltage and distance between the capillary and the roller and the specified flow of the solution on the feeding pump and after a certain time the layer covering the roller with thickness of 1-100 μm is obtained. Thereafter the inner intermediate layer of polycarbonate silicone is formed by electrospraying. When the thickness of the layer is approximately 5 to 100 μm the process is stopped and stents are placed on the formed layer and similarly like applying the former intermediate layer the application of the inner intermediate layer is continued on the whole length of the roller. Thereafter the final surface layer is applied like the first surface layer until a prefabricated unit with the polymer material thickness from 50 to 250 μm is obtained.

The present invention provides a preparation method preparation method for three-layer artificial blood vessel and application thereof. The three-layer artificial blood vessel comprise three layers, electrospinning inner layer, dense middle layer and electrospinning outer layer, the three-layer structure is closely combined and difficult to separate. The inner layer with a cytoskeleton-like structure can promote the formation of intima; the dense middle layer can effectively prevent the leakage of biomacromolecules and increase the puncture resistance of the whole artificial blood vessel; and the outer layer can promote the growth of tissue cells and better integrate with tissue. The three-layer artificial blood vessel provided by the invention has excellent blood compatibility, good flexibility, good puncture resistance and interlayer peeling resistance. The preparation method is convenient and is suitable for industrial scale production.