The present invention, in which RPE cells are suspended in a medium pharmaceutically acceptable as an ocular irrigating/washing solution and containing a poloxamer, achieves improvement of the post-thawing survival rate of cryopreserved RPE cells, improvement of the photoreceptor cell protection effect by RPE cell transplanted immediately after thawing, and prevention of loss of RPE cells in various steps from thawing to transplantation.

The present invention, in which RPE cells are suspended in a medium pharmaceutically acceptable as an ocular irrigating/washing solution and containing a poloxamer, achieves improvement of the post-thawing survival rate of cryopreserved RPE cells, improvement of the photoreceptor cell protection effect by RPE cell transplanted immediately after thawing, and prevention of loss of RPE cells in various steps from thawing to transplantation.

Various aspects and embodiments disclosed herein relate generally to the stem cell biology and cell replacement therapy. Embodiments include compositions and methods for modelling, treatment, reducing resistance to the treatment, prevention, and diagnosis of a condition/disease associated with retinal degenerative diseases or a related clinical condition thereof. Other embodiments include methods and compositions for developing pluripotent stem cell-derived 3D tissues.

Various aspects and embodiments disclosed herein relate generally to the stem cell biology and cell replacement therapy. Embodiments include compositions and methods for modelling, treatment, reducing resistance to the treatment, prevention, and diagnosis of a condition/disease associated with retinal degenerative diseases or a related clinical condition thereof. Other embodiments include methods and compositions for developing pluripotent stem cell-derived 3D tissues.

A method of treating medical conditions by stem cell therapy. Stem cells are transplanted into and onto a human or animal patient in need, Then stem cell proliferation is increased, activating migration to sites of inflammation and epigenetic effects, regenerating damaged tissues through inhibition of apoptosis, inhibition of inflammation and oxidative stress, and inducing angiogenesis and stem cell differentiation into various cells by administering substances that induce GSK-3 beta inhibition and HDAC inhibition.

A method of treating medical conditions by stem cell therapy. Stem cells are transplanted into and onto a human or animal patient in need, Then stem cell proliferation is increased, activating migration to sites of inflammation and epigenetic effects, regenerating damaged tissues through inhibition of apoptosis, inhibition of inflammation and oxidative stress, and inducing angiogenesis and stem cell differentiation into various cells by administering substances that induce GSK-3 beta inhibition and HDAC inhibition.

A bone augmentation composition and a method for making the bone augmentation. The method including hydrating an allograft comprising cortical fibers with a cell culture media, seeding the hydrated allograft with a solution of human stem cells, and culturing the stem-cell seeded allograft to grow a population of the stem cells in the seeded graft prior to freezing the bone augmentation composition for storage.

A method of treating acute respiratory distress syndrome (ARDS) in a subject in need thereof is provided. The method comprising administering to the subject a composition comprising a therapeutically effective amount of astrocytes, thereby treating the ARDS.

A method of treating acute respiratory distress syndrome (ARDS) in a subject in need thereof is provided. The method comprising administering to the subject a composition comprising a therapeutically effective amount of astrocytes, thereby treating the ARDS.

The present disclosure features methods and compositions for treating Alzheimer's disease. The disclosed methods comprise administering to a subject having or suspected of having Alzheimer's a hematopoietic stem progenitor cell expressing at least one neuroprotective agent, such as ApoE2, Trem2, and/or a metallothionein.