In some aspects, disclosed herein are methods and compositions for treatment of inflammatory bowel disease using fibroblasts or derivatives thereof. Disclosed herein are compositions having tolerogenic properties. Compositions of the present disclosure include fibroblasts, activated fibroblasts, fibroblast apoptotic bodies, and fibroblast exosomes. Methods of the present disclosure include, in some cases, providing fibroblasts or derivatives thereof to a subject to treat an inflammatory bowel disease. In some cases, dendritic cells are cultured with fibroblasts and provided to a subject to treat an inflammatory bowel disease.

In some aspects, disclosed herein are methods and compositions for treatment of inflammatory bowel disease using fibroblasts or derivatives thereof. Disclosed herein are compositions having tolerogenic properties. Compositions of the present disclosure include fibroblasts, activated fibroblasts, fibroblast apoptotic bodies, and fibroblast exosomes. Methods of the present disclosure include, in some cases, providing fibroblasts or derivatives thereof to a subject to treat an inflammatory bowel disease. In some cases, dendritic cells are cultured with fibroblasts and provided to a subject to treat an inflammatory bowel disease.

Materials and methods for preparing a hydrogel are disclosed. Specifically, two separate solutions A and B, of which the solution A comprises enzyme horseradish peroxidase and the solution B comprises hydrogen peroxide is provided. At least one of solution A or B comprises calcium ions in the form of a pharmaceutically acceptable salt, and further the solution A and/or the solution B comprises hydroxyphenyl derivative of hyaluronan of a particular formula. A hydrogel based on hydroxyphenyl derivative of hyaluronan and a method of preparing and using the same is also disclosed.

Materials and methods for preparing a hydrogel are disclosed. Specifically, two separate solutions A and B, of which the solution A comprises enzyme horseradish peroxidase and the solution B comprises hydrogen peroxide is provided. At least one of solution A or B comprises calcium ions in the form of a pharmaceutically acceptable salt, and further the solution A and/or the solution B comprises hydroxyphenyl derivative of hyaluronan of a particular formula. A hydrogel based on hydroxyphenyl derivative of hyaluronan and a method of preparing and using the same is also disclosed.

Some embodiments of the invention include modified cells. Certain embodiments of the invention include methods of using modified cells. Other embodiments of the invention include methods of administering modified cells. Further embodiments of the invention include methods of administering modified cells to treat diseases. Additional embodiments of the invention are also discussed herein.

Some embodiments of the invention include modified cells. Certain embodiments of the invention include methods of using modified cells. Other embodiments of the invention include methods of administering modified cells. Further embodiments of the invention include methods of administering modified cells to treat diseases. Additional embodiments of the invention are also discussed herein.

In some aspects, disclosed herein are methods and compositions for treatment of sclerosing cholangitis using fibroblasts or derivatives thereof. The disclosed compositions include fibroblasts, engineered fibroblasts, exosomes obtained from fibroblasts, and conditioned media derived from fibroblasts. Methods of the present disclosure include providing fibroblasts to a subject to treat sclerosing cholangitis in the subject. Fibroblasts of the disclosure include fibroblasts capable of reducing inflammation in a subject. In certain aspects, fibroblasts are cultured with activating agents prior to therapeutic administration.

In some aspects, disclosed herein are methods and compositions for treatment of sclerosing cholangitis using fibroblasts or derivatives thereof. The disclosed compositions include fibroblasts, engineered fibroblasts, exosomes obtained from fibroblasts, and conditioned media derived from fibroblasts. Methods of the present disclosure include providing fibroblasts to a subject to treat sclerosing cholangitis in the subject. Fibroblasts of the disclosure include fibroblasts capable of reducing inflammation in a subject. In certain aspects, fibroblasts are cultured with activating agents prior to therapeutic administration.

In some aspects, disclosed herein are methods and compositions for treatment of sclerosing cholangitis using fibroblasts or derivatives thereof. The disclosed compositions include fibroblasts, engineered fibroblasts, exosomes obtained from fibroblasts, and conditioned media derived from fibroblasts. Methods of the present disclosure include providing fibroblasts to a subject to treat sclerosing cholangitis in the subject. Fibroblasts of the disclosure include fibroblasts capable of reducing inflammation in a subject. In certain aspects, fibroblasts are cultured with activating agents prior to therapeutic administration.

The disclosure provides means, methods, and compositions of preventing, reducing, and treating kidney failure through the administration of fibroblasts, modified fibroblasts, and/or products derived from fibroblasts. The disclosure may also concern administration of fibroblasts prior to, concurrent with, or subsequent to administration of a nephrotoxic agent results in production of renal function. In some embodiments, fibroblasts are enhanced for augmentation of nephron-regenerative properties through culture under means including hypoxia, histone deacetylase inhibitor treatment, oxytocin, and/or DNA methyltransferase inhibitors.