Disclosed herein are methods for the preparation of allergenic extracts. In one embodiment, the methods are carried out without having to adjust the pH of the extraction phase. The methods provide efficient recovery of allergenic extract from allergenic source material.

Disclosed herein are methods for the preparation of allergenic extracts. In one embodiment, the methods are carried out without having to adjust the pH of the extraction phase. The methods provide efficient recovery of allergenic extract from allergenic source material.

The present disclosure provides a preparation method of a thermosensitive gel for treating androgenetic alopecia. The preparation method includes the following steps: step 1, separating umbilical cord mesenchymal stem cells (UC-MSCs); step 2, conducting adherent culture of the UC-MSCs; step 3, preparing a lyophilized powder of umbilical cord-mesenchymal stem cell-conditioned medium (UC-MSC-CM) supernatant; and step 4, preparing the thermosensitive gel. In the present disclosure, the thermosensitive gel is prepared by using the UC-MSC-CM supernatant. Based on an important role of cytokines encapsulated in exosomes secreted by the UC-MSCs in the hair growth process, the exosomes are encapsulated in the thermosensitive gel for topical use in treating androgenetic alopecia, featuring easy operation, convenient treatment, and excellent efficacy.

Some embodiments are directed to a method for preparing a skin substitute, a dermal substitute, to a skin substitute, to a dermal substitute and to a kit for implementing the method. Some other embodiments are directed to a graft that can consist of of a skin substitute and to the use thereof as treating a skin disorder and/or a loss of skin substance.

Some embodiments are directed to a method for preparing a skin substitute, a dermal substitute, to a skin substitute, to a dermal substitute and to a kit for implementing the method. Some other embodiments are directed to a graft that can consist of of a skin substitute and to the use thereof as treating a skin disorder and/or a loss of skin substance.

There is provided a porous double-network hydrogel comprising: a first network comprising a first polymer; a second network comprising a second polymer. The porous double-network hydrogel comprises pores having a diameter of at least 1 μm, and the porous double-network hydrogel is perfusable and injectable.

There is provided a porous double-network hydrogel comprising: a first network comprising a first polymer; a second network comprising a second polymer. The porous double-network hydrogel comprises pores having a diameter of at least 1 μm, and the porous double-network hydrogel is perfusable and injectable.

The present invention involves implants suitable for surgical implantation into subjects. In some embodiments the implants comprise a biocompatible scaffold material and blood vessels containing engineered endothelial cells—such as E4ORF1+ engineered endothelial cells or engineered endothelial cells that express certain marker molecules. The present invention provides implants, methods for preparing such implants, and methods of treatment utilizing such implants.

The present invention involves implants suitable for surgical implantation into subjects. In some embodiments the implants comprise a biocompatible scaffold material and blood vessels containing engineered endothelial cells—such as E4ORF1+ engineered endothelial cells or engineered endothelial cells that express certain marker molecules. The present invention provides implants, methods for preparing such implants, and methods of treatment utilizing such implants.

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.