A dual-modality nanoprobe targeting glioblastoma and preparation method thereof are described. The dual-modality nanoprobe of the present disclosure comprises DSPE-PEG(2000)-Amine, superparamagnetic iron oxide nanoparticles (SPIONs), Cy7-NHS molecule, targeting polypeptide and/or trans-mirror structure (i.e., enantiomer) thereof. The dual-modality nanoprobe can integrate the advantages of magnetic resonance and fluorescence imaging and thus provide clearer anatomical structure information of brain tumors; in the imaging process, the dual-modality nanoprobe can not only provide clearer image results, but also can specifically identify target sites. Also, the dual-modality nanoprobe of the present disclosure also has good in vivo stability.

A liposomal composition (“ADx-003”) is provided, ADx-003 comprising a first phospholipid; a sterically bulky excipient that is capable of stabilizing the liposomal composition; a second phospholipid that is derivatized with a first polymer; a macrocyclic gadolinium-based imaging agent; and a third phospholipid that is derivatized with a second polymer, the second polymer being conjugated to a targeting ligand, the targeting ligand being represented by Formula I: ##STR00001##
wherein X is —CH.sub.2—, —CH.sub.2—CH.sub.2—, —CHO—, or —O—CO—; Y is —CH—CH═CH— or ##STR00002##
A and B are independently selected from C and N; R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are independently selected from —H, halogen, —OH, and —CH.sub.3; and R.sub.5, R.sub.6, and R.sub.7 are independently selected from —H, halogen, —OH, —OCH.sub.3, —NO.sub.2, —N(CH.sub.3).sub.2, C.sub.1-C.sub.6 alkyl, or a substituted or unsubstituted C.sub.4-C.sub.6 aryl group, except that when A and/or B is N the adjacent R.sub.5 and/or R.sub.7 is —H, or a pharmaceutically acceptable salt thereof.

The present system relates to a mains-powerable, compact and customizable alternating current magnetic field plate for magnet-assisted transfection of genes to target cells. Magnet plate is based on at least one alternating current electromagnet comprising a laminated steel stack core and a multi-layer, multi-turn coil wound longitudinally therearound. The system includes a voltage rating adjustment controller, as well as a current adjuster for selective control of magnetic force applied to genetic material for delivery. Rapid magnetic field polarity switching exacts lateral motion efficiently and uniformly, thus improving the distribution of means, such as SPIONS, used to transfect cells with genes of interest, and in turn enhancing gene delivery and tissue localization, especially for hard-to-transfect genes, compared to DC magnet plates.

The invention relates to a polymer comprising a crosslinked matrix, the matrix being based on at least: a) 20 to 90% hydrophilic monomer; b) 5 to 50% radio-opaque halogenated monomer; c) 1 to 15% non-biodegradable hydrophilic crosslinking agent; and d) 0.1 to 10% transfer agent chosen among the alkyl halides and cycloaliphatic or aliphatic thiols having, in particular, 2 to 24 carbon atoms, and optionally having another functional group chosen among the amino, hydroxy and carboxy groups. The invention further relates to a pharmaceutical composition comprising at least one polymer according to the invention, in association with a pharmaceutically acceptable vehicle, advantageously for a parenteral administration. The invention further relates to a kit comprising a pharmaceutical composition comprising the polymer according to the invention in association with a pharmaceutically acceptable vehicle for a parenteral administration, and an injection means.

There are described magnetic nanoparticles the surface of which is functionalized with catechol and constructs comprising a plurality of said nanoparticles encapsulated in a biocompatible polymer matrix, wherein a molecule with therapeutic action is optionally dispersed, said polymer matrix optionally being in turn further functionalized; there are further described cells of the immune system incorporating said polymeric constructs giving rise to their engineering.

The disclosure relates to methods for treating tumors. In particular, the disclosure relates to a method of treating a tumor by magnetic resonance image-guided radiation therapy in a subject in need thereof, said method comprising the steps of: (i) administering an efficient amount of high-Z element containing nanoparticles having, contrast enhancement for magnetic resonance imaging and/or radiosensitizing properties for radiation therapy, in a subject in need thereof, and, (ii) exposing said subject to magnetic resonance image-guided radiation therapy by means of a Magnetic Resonance Imaging Guided Linear Accelerator (MR-Linac),
wherein said high-Z element containing nanoparticles are nanoparticles containing an element with an atomic Z number higher than 40, preferably higher than 50, and said nanoparticles have a mean hydrodynamic diameter below 20 nm, for example between 1 and 10 nm, preferably between 2 and 8 nm.

Brain-targeted (BT) and ROS-activable nanoconstructs (NC) comprising a metal oxide nanoparticle embedded in a matrix of lipid and a brain targeted polymer (BTP)/platform configured to facilitate blood brain barrier (BBB) penetration and accumulation in a disease area of the central nervous system (CNS), as well as compositions having the BT and ROS-activable NC and methods of using the BT and ROS-activable NC to treat and diagnose a CNS disease or condition.

Provided is a drug useful for photoimmunotherapy. Specifically, provided is a conjugate including an antibody molecule, a particle having an average particle diameter of 100 nm or less, and a photosensitive portion. In the conjugate, the particle having an average particle diameter of 100 nm or less is linked to the antibody molecule, and at least one of the antibody molecule or the particle is bound to the photosensitive portion. The photosensitive portion is a portion showing increase of hydrophobicity when irradiated with a light beam having a wavelength of from 500 nm to 900 nm or a portion containing a phthalocyanine skeleton.

A magnetomotive imaging probe device and method is described. The probe device includes a housing having an outer surface and an inner cavity; a magnet; a sensing device configured to detect distance, movement, or magnetic material. The magnet is arranged in the inner cavity of the housing and the sensing device on the outer surface of the housing, and the magnet is arranged to generate a time-varying magnetic field at an imaging plane of the sensing device. The magnet is intended to move magnetic nanoparticles in tissue such that a movement can be detected with the sensing device (ultrasound, optical or other). The detected motion infers the presence of magnetic material (nanoparticles).

Provided herein are compositions comprising a drug delivery system comprising a phospholipid that is hydrolyzed by phospholipase A2 (PLA2) enzyme and a drug. Also provided herein are methods for treating or for determining the location of a region to be treated or monitored in a subject in need thereof, the methods comprising: administering to the subject the disclosed composition.