A soluble, lyophilized coffee has improved properties compared to known lyophilized coffee and is obtained by a dehydration process implementing a cryogenic step under pressure followed by a lyophilization step.

The present invention provides a cosmetic shaped product: which is substantially formed with relatively low-molecular-weight hyaluronic acid or a water-soluble salt thereof having a high penetrability into the skin; which is easily soluble to water; and which has satisfactory handling properties, and a method for producing the cosmetic shaped product. There is provided a cosmetic shaped product being a sponge-like shaped body formed with at least one polyanionic polysaccharide of hyaluronic acid and a water-soluble salt thereof each having an average molecular weight of 30,000 or lower, the sponge-like shaped body having a bulk specific gravity of 5 to 500 mg/mL. This cosmetic shaped product can be produced by freeze-drying an aqueous solution having a content of the polyanionic polysaccharide of 0.5 to 30% by mass.

The present invention provides methods of administering a composition comprising hyaluronic acid to a subject's skin, comprising providing an effective amount of hyaluronic acid in solid form; constituting the hyaluronic acid in an aqueous solution; and administering the solution comprising hyaluronic acid to the subject's skin.

The present invention provides a method for preparing an high internal phase of pumpkin seed protein nanoparticles, including dissolving pumpkin seed protein powder in water and adjusting pH to approximately 8 to 11 to obtain a pumpkin seed protein solution; adding the pumpkin seed protein solution through a peristaltic pump into an ethanol solution with stirring for 4 hours to obtain a first solution; performing a centrifugation to the first solution and collecting precipitates; diluting the precipitates with water and performing a freeze-drying to obtain the pumpkin seed protein nanoparticles. The present invention first uses an anti-solvent method to prepare pumpkin seed protein nanoparticles, which have excellent emulsifying properties and can stabilize the high internal phase emulsion. In addition, the resulting pumpkin seed protein nanoparticles can stabilize the high internal phase emulsion independently without compounding with other substances.

Business methods, processes and systems for collection, storage and distribution of a biological sample material for producing a cosmetic formulation product.

One aspect of the invention provides a method for freeze-drying surfactant-stabilized microbubbles. The method includes: preparing vials comprising a mixture comprising microbubbles; partially submerging the vials in a chilled water bath, wherein the water bath has a sub-freezing temperature; placing the vials on a cooled shelf of a lyophilizer; freeze-drying the vials in the lyophilizer; and capping the freeze-dried vials. Another aspect of the invention provides a method for annealing surfactant-stabilized microbubbles. The method includes: preparing vials comprising a mixture comprising microbubbles; passing the vials in and out of liquid nitrogen (LN.sub.2) until the mixture is frozen; holding the vials at −20° C.; placing the vials on a cooled shelf of a lyophilizer; freeze-drying the vials in the lyophilizer; and capping the freeze-dried vials.

A method for enhancing skin moisture of a subject in need thereof includes administering to the subject a composition including a white roselle (Hibiscus sabdariffa cv.) extract. The white roselle extract is obtained by lysing a cell wall of a white roselle calyx by ice crystals and extracting the lysed white roselle calyx with a solvent.

Disclosed are a method for preparing exosomes from bovine placenta, lyophilized powder of the exosomes, as well as a composition containing exosomes from animal placenta and a preparation method and use thereof in anti-wrinkle on skin.

A method for manufacturing a fucoidan cosmetic composition includes: washing mozuku to eliminate residual salt and impurities; adding citric acid to the washed mozuku and pulverizing the mozuku; adding water to the pulverized mozuku and performing reflux extraction to obtain a high molecular weight fucoidan; adding a neutralizing agent to the high molecular weight fucoidan for neutralization; adding diatomite to the neutralized high molecular weight fucoidan and causing a reaction for 30 minutes for deodorization; filtering the deodorized high molecular weight fucoidan to eliminate insoluble substances; adding a lactic acid bacterium to the high molecular weight fucoidan removed of the insoluble substances and causing fermentation to obtain a low molecular weight fucoidan; concentrating the purified low molecular weight fucoidan with a reduced pressure evaporator; and powdering the concentrated low molecular weight fucoidan through freeze drying.

A composition including a mixture of Trigonella foenum-graecum extract and bentonite. The mixture is applicable in several technological fields, including the pharmacological, cosmetic, and veterinary field. Notably, the composition is useful in the treatment of a viral infection. The composition is synergistic in the sense that the mixture of Trigonella foenum-graecum extract and bentonite has a higher efficiency than each of the components alone.