Described herein are fluid complex coacervates that produce solid adhesives in situ. Oppositely charged polyelectrolytes were designed to form fluid adhesive complex coacervates at ionic strengths higher than the ionic strength of the application site, but an insoluble adhesive solid or gel at the application site. When the fluid, high ionic strength adhesive complex coacervates are introduced into the lower ionic strength application site, the fluid complex coacervate is converted to a an adhesive solid or gel as the salt concentration in the complex coacervate equilibrates to the application site salt concentration. In one embodiment, the fluid complex coacervates are designed to solidify in situ at physiological ionic strength and have numerous medical applications. In other aspects, the fluid complex coacervates can be used in aqueous environment for non-medical applications.

Described herein are fluid complex coacervates that produce solid adhesives in situ. Oppositely charged polyelectrolytes were designed to form fluid adhesive complex coacervates at ionic strengths higher than the ionic strength of the application site, but an insoluble adhesive solid or gel at the application site. When the fluid, high ionic strength adhesive complex coacervates are introduced into the lower ionic strength application site, the fluid complex coacervate is converted to a an adhesive solid or gel as the salt concentration in the complex coacervate equilibrates to the application site salt concentration. In one embodiment, the fluid complex coacervates are designed to solidify in situ at physiological ionic strength and have numerous medical applications. In other aspects, the fluid complex coacervates can be used in aqueous environment for non-medical applications.

A preparation method of a hemostatic material is provided, wherein the method mainly includes mixing a keratin and an alginate; obtaining a keratin-alginate composite scaffold by a freeze-gelation method; and drying the keratin-alginate composite scaffold to obtain a hemostatic material. Further, a methylene blue can be loaded into the hemostatic material so that the hemostatic material has antimicrobial photodynamic abilities.

A preparation method of a hemostatic material is provided, wherein the method mainly includes mixing a keratin and an alginate; obtaining a keratin-alginate composite scaffold by a freeze-gelation method; and drying the keratin-alginate composite scaffold to obtain a hemostatic material. Further, a methylene blue can be loaded into the hemostatic material so that the hemostatic material has antimicrobial photodynamic abilities.

A powder spray device includes a funnel member, a first three-way joint, an air-current supply tube, a discharge tube, a vibration motor, a bypass air-current tube, and a switching mechanism. The first three-way joint has a first opening connected to an outlet at a lower end of the funnel member. The air-current supply tube and discharge tube are respectively connected to second and third openings of the first three-way joint. The vibration motor is fixed onto an outer surface of a funnel body of the funnel member. The bypass air-current tube branches off from the air-current supply tube and is connected to the discharge tube. The switching mechanism switches from and to standby state, in which compressed gas is sent only through the bypass air-current tube, to and from spray-coating state, in which compressed gas is sent out through the air-current supply tube, and also through the bypass air-current tube.

A powder spray device includes a funnel member, a first three-way joint, an air-current supply tube, a discharge tube, a vibration motor, a bypass air-current tube, and a switching mechanism. The first three-way joint has a first opening connected to an outlet at a lower end of the funnel member. The air-current supply tube and discharge tube are respectively connected to second and third openings of the first three-way joint. The vibration motor is fixed onto an outer surface of a funnel body of the funnel member. The bypass air-current tube branches off from the air-current supply tube and is connected to the discharge tube. The switching mechanism switches from and to standby state, in which compressed gas is sent only through the bypass air-current tube, to and from spray-coating state, in which compressed gas is sent out through the air-current supply tube, and also through the bypass air-current tube.

Compositions and methods related to powdered hemostats that crosslink during and/or after application to a bleeding site are described. The compositions may comprise a first component comprising a polyalkylene oxide-based polymer functionalized with electrophilic reactive groups, and a second component that comprises a protein such as albumin and an optional crosslinking initiator—which may be a base or basic salt such as sodium bicarbonate. The compositions may in certain applications act as hemostats when applied in dry powder form to a bleeding wound, whereupon the first component and the second component of the composition crosslink to form a hydrogel.

Compositions and methods related to powdered hemostats that crosslink during and/or after application to a bleeding site are described. The compositions may comprise a first component comprising a polyalkylene oxide-based polymer functionalized with electrophilic reactive groups, and a second component that comprises a protein such as albumin and an optional crosslinking initiator—which may be a base or basic salt such as sodium bicarbonate. The compositions may in certain applications act as hemostats when applied in dry powder form to a bleeding wound, whereupon the first component and the second component of the composition crosslink to form a hydrogel.

A method of production of a tissue sealing patch is disclosed. The method comprises applying a vacuum to a heated work surface; applying a solution of a biocompatible polyurethane polymer to the work surface and spreading it over the work surface with a polymer blade; evaporating the solvent; heating the work surface above the softening temperature of the polymer; spreading powdered tissue sealant material over the polymer film; incorporating the tissue sealant material to a depth of 20-60 μm in the film by pressing on a release sheet placed over the powder and polymer film; removing the release sheet from the adhesive patch material; releasing the vacuum; cooling said work surface; and removing the adhesive patch material from said work surface. The biocompatible polymer preferably comprises PEG-caprolactone-lactic acid units connected by urethane linkages, the PEG having a molecular weight of 3000-3500 amu, and a CL:LA:PEG ratio of 34:2:1.

The present invention relates to a composition for hemostasis which contains collagen, stabilizer, and thrombin, and a container including the same. The present invention is applicable to a bleeding patient requiring emergency treatment with a simple method of use. There is no toxicity and no problem of blood infection. A biodegradation rate is fast. In this regard, the present invention achieves an excellent hemostatic effect. Therefore, the composition for hemostasis is useful as a hemostat.