Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Disclosed herein are methods of producing a cartilaginous implant by producing a polymer scaffold composition by electrospinning a polymer solution onto a collector in order to obtain polymer fibers; crosslinking the polymer fibers; and adding a plurality of cells to the polymer scaffold composition, wherein the plurality of cells comprises cartilaginous cells to form a cartilaginous implant.

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Provided herein are biocompatible and/or biodegradable hydrogel compositions comprising native collagen and chondroitin sulfate, the collagen and chondroitin sulfate being chemically cross-linked thereby forming a matrix. The native collagen may comprise recombinant human collagen type I (rHCI), recombinant human collagen type III (rHCIII), or a combination thereof, for example. Methods and uses thereof for regeneration or repair of tissue, improvement of tissue function, mechanical stabilization of tissue, prevention of tissue damage, or prevention of tissue loss of function are described, particularly with respect to cardiac tissue and myocardial infarction events.

Provided herein are biocompatible and/or biodegradable hydrogel compositions comprising native collagen and chondroitin sulfate, the collagen and chondroitin sulfate being chemically cross-linked thereby forming a matrix. The native collagen may comprise recombinant human collagen type I (rHCI), recombinant human collagen type III (rHCIII), or a combination thereof, for example. Methods and uses thereof for regeneration or repair of tissue, improvement of tissue function, mechanical stabilization of tissue, prevention of tissue damage, or prevention of tissue loss of function are described, particularly with respect to cardiac tissue and myocardial infarction events.

Provided herein are biocompatible and/or biodegradable hydrogel compositions comprising native collagen and chondroitin sulfate, the collagen and chondroitin sulfate being chemically cross-linked thereby forming a matrix. The native collagen may comprise recombinant human collagen type I (rHCI), recombinant human collagen type III (rHCIII), or a combination thereof, for example. Methods and uses thereof for regeneration or repair of tissue, improvement of tissue function, mechanical stabilization of tissue, prevention of tissue damage, or prevention of tissue loss of function are described, particularly with respect to cardiac tissue and myocardial infarction events.

The present solution can temporarily impart the handling characteristics of corneal stroma to the otherwise very thin, flimsy, coiling, and fragile Descemet membrane endothelial keratoplasty (DMEK) tissue during its insertion into the anterior chamber and positioning in apposition against the cornea of the recipient eye. The device of the present solution can be configured in a number of ways. In a first configuration, a scaffold can be coupled with the endothelial side of the DMEK graft. In a second configuration, the scaffold can be coupled with the stromal side of the DMEK graft. In a third configuration, one or more scaffolds can be coupled with both the endothelial and stromal side of the DMEK graft.

The present solution can temporarily impart the handling characteristics of corneal stroma to the otherwise very thin, flimsy, coiling, and fragile Descemet membrane endothelial keratoplasty (DMEK) tissue during its insertion into the anterior chamber and positioning in apposition against the cornea of the recipient eye. The device of the present solution can be configured in a number of ways. In a first configuration, a scaffold can be coupled with the endothelial side of the DMEK graft. In a second configuration, the scaffold can be coupled with the stromal side of the DMEK graft. In a third configuration, one or more scaffolds can be coupled with both the endothelial and stromal side of the DMEK graft.

Artificial cartilage materials for repair and replacement of cartilage, such as load-bearing and articular cartilage. The artificial cartilage materials can include a hydrogel with an internal polymer support network that impart the hydrogel mechanical properties similar to that of natural cartilage. In some examples, the hydrogels include a cross-linked cellulose network and a double network of polyvinyl alcohol (PVA) and polyacrylamide-methyl propyl sulfonic acid (PAMPS) polymers. The hydrogels may include specific formulations of different polymers to impart mechanical properties that are within a cartilage equivalent range. The artificial cartilage materials may include a porous base that is bonded to the hydrogel for interfacing with surrounding tissues and promoting ingrowth of bone and/or cartilage. Thus, the materials may be well suited for forming a synthetic graft, such as an osteochondral graft, for implantation into a patient's body.