Tissue fillers derived from decellularized tissues are provided. The tissue fillers can include acellular tissue matrices that have reduced inflammatory responses when implanted in a body. Also provided are methods of making and therapeutic uses for the tissue fillers.

Tissue fillers derived from decellularized tissues are provided. The tissue fillers can include acellular tissue matrices that have reduced inflammatory responses when implanted in a body. Also provided are methods of making and therapeutic uses for the tissue fillers.

Disclosed are intra-articular compositions comprising at least one low-molecular-weight linear hyaluronic acid or hyaluronate and at least one high-molecular-weight linear hyaluronic acid or hyaluronate, said compositions being characterised by a dynamic viscosity ranging between 10 and 60 Pa*s (at 0.01 s.sup.-1), a crossover frequency ranging between 1 and 10 rad/s, and a value of the viscous and elastic moduli at the crossover frequency ranging between 20 and 110 Pa.

Disclosed are intra-articular compositions comprising at least one low-molecular-weight linear hyaluronic acid or hyaluronate and at least one high-molecular-weight linear hyaluronic acid or hyaluronate, said compositions being characterised by a dynamic viscosity ranging between 10 and 60 Pa*s (at 0.01 s.sup.-1), a crossover frequency ranging between 1 and 10 rad/s, and a value of the viscous and elastic moduli at the crossover frequency ranging between 20 and 110 Pa.

Disclosed are intra-articular compositions comprising at least one low-molecular-weight linear hyaluronic acid or hyaluronate and at least one high-molecular-weight linear hyaluronic acid or hyaluronate, said compositions being characterised by a dynamic viscosity ranging between 10 and 60 Pa*s (at 0.01 s.sup.-1), a crossover frequency ranging between 1 and 10 rad/s, and a value of the viscous and elastic moduli at the crossover frequency ranging between 20 and 110 Pa.

A preparation method of a hydrogel of a mercapto-modified macromolecular compound includes the steps of combining the mercapto-modified macromolecular compound with an acrylated macromolecular compound and/or an acrylated micromolecular crosslinker. The mercapto-modified macromolecular compound can be crosslinked with the acrylated macromolecular compound and/or the acrylated micromolecular crosslinker under physiological conditions to form the hydrogel. Due to the rapid mercapto-vinyl crosslinking reaction, the formed hydrogel system can be quickly gelled in situ after being injected into the body. The hydrogel is thus suitable for use in the fields of biomedicine, medical cosmetic plastic surgery and cosmetics.

A preparation method of a hydrogel of a mercapto-modified macromolecular compound includes the steps of combining the mercapto-modified macromolecular compound with an acrylated macromolecular compound and/or an acrylated micromolecular crosslinker. The mercapto-modified macromolecular compound can be crosslinked with the acrylated macromolecular compound and/or the acrylated micromolecular crosslinker under physiological conditions to form the hydrogel. Due to the rapid mercapto-vinyl crosslinking reaction, the formed hydrogel system can be quickly gelled in situ after being injected into the body. The hydrogel is thus suitable for use in the fields of biomedicine, medical cosmetic plastic surgery and cosmetics.

A malleable polysaccharide soft tissue filler for filling tissue cavities or voids such as those resulting from tumor removal or other tissue resection. The soft tissue filler includes a first cross-linking polysaccharide, preferably a beta-D glucan and a second cross-linking polysaccharide. The soft tissue filler is both porous and malleable and can be formed to accommodate the tissue cavity or void. The soft tissue filler can include an embedded marker for locating on medical imaging. Methods of making the soft tissue filler including lyophilizing an aqueous polysaccharide suspension are disclosed. The second cross-linking polysaccharide provides for increased structural integrity in a high-porosity and malleable soft tissue filler in which the respective cross-linking polysaccharides synergistically provide structural scaffolding for one another. Methods of use are also disclosed.

A malleable polysaccharide soft tissue filler for filling tissue cavities or voids such as those resulting from tumor removal or other tissue resection. The soft tissue filler includes a first cross-linking polysaccharide, preferably a beta-D glucan and a second cross-linking polysaccharide. The soft tissue filler is both porous and malleable and can be formed to accommodate the tissue cavity or void. The soft tissue filler can include an embedded marker for locating on medical imaging. Methods of making the soft tissue filler including lyophilizing an aqueous polysaccharide suspension are disclosed. The second cross-linking polysaccharide provides for increased structural integrity in a high-porosity and malleable soft tissue filler in which the respective cross-linking polysaccharides synergistically provide structural scaffolding for one another. Methods of use are also disclosed.

A method for incorporating at least one organic compound within a hydrogel, wherein the incorporation is carried out by dialysis, the organic compound being solubilized in the dialysis solution at a concentration equal to or greater than the target concentration.