Provided herein are tissue grafts, and in particular human placenta-derived tissue grafts and methods and articles for the manufacture and use thereof.

A medical support device (1), for use in a woman's breast as an implant without incapsulated material, wherein the medical support device (1) comprises at least a wall shaped portion (2) having a first side (3), which shows a bulge out of a plane (4), and a second side (5) of the wall shaped portion (2) has a corresponding cavity, for example a dome shape, wherein the medical support device (1) comprises an elastomer. The device will keep endogenous tissue and/or fat and/or added material, such as hyaluronic acid, in place inside of deep gland tissue of a woman's breast by means of being attached to tissue, such as muscle. A method of producing and to use such a medical support device (1).

Described herein are dehydrated, laminated tissue grafts composed of two or more membrane layers of an amnion membrane, a chorion membrane, a Wharton's jelly, or an intermediate tissue layer, where a layer of micronized placental tissue is interposed between two of the layers. Also described herein are methods for making and using the laminated tissue grafts.

A solubilized notochordal cell matrix powder dissolved in a carrier solvent or formed as a gel is provided. The notochordal cell matrix powder originates from lyophilized and treated porcine nucleus pulposus tissue containing notochordal cells. The powder contains less than 20% of porcine nucleid acids, and the powder contains a substantially unchanged amount of porcine protein content compared to the originating porcine nucleus pulposus tissue. The solubilized notochordal cell matrix powder is capable of stimulating native or stem cells to proliferate and produce a significant increase inglycosaminoglycansand type-II collagen matrix. Embodiments of the invention can be used for the disc regenerative treatment of discogenic back and neck pain in an orthopaedic and/or pharmaceutical setting/approach.

A graft compression system for compressing soft tissue grafts used in connection with reconstructive surgery on the anterior cruciate ligament (ACL). The graft compression system includes a compression chamber having an elongate hollow shaft body having two ends that are threaded to mate with correspondingly threaded collet nuts. Collets are removably inserted into, and engage, the collet nuts fastened to opposing ends of the compression chamber A surgical graft may be inserted into a hollow compression tube having a lumen with a compressible diameter, said compression tube being sized for insertion into the collets and compression chamber. When such collet nuts are tightened by a user of the graft compression system, the inner diameters of the respective collet nuts nested within such collet nuts are decreased, causing the diameter of the lumen of the hollow compression tube to in turn be decreased and compress the surgical graft within.

A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.

Current approaches in small diameter vascular grafts for coronary artery bypass surgeries fail to address physiological variations along the graft that contribute to thrombus formation and ultimately graft failure. An interlayer drug delivery system can sustain delivery of heparin through the graft with a high degree of temporal and spatial control. A heparin-loaded gelatin methacrylate interlayer sits between a biohybrid composed of decellularized bovine pericardium and poly(propylene fumarate) and UV crosslinking is controlled via 3D printed shadow masks. The masks enable control of the resultant gelMA crosslinking and properties by modulating the incident light intensity on the graft. High doses of heparin have detrimental effects on endothelial cell function. When exposed to heparin in a slower, more sustained manner consistent with the masks, endothelial cells behave similarly to untreated cells. Slower release profiles cause significantly more release of tissue factor pathway inhibitor, an anticoagulant, than a faster release profile.

Described herein are tissue grafts derived from the placenta with improved physical and biological properties. In one aspect, the tissue graft includes a first membrane comprising Wharton's jelly laminated with amnion, chorion, or a combination thereof. The presence of Wharton's jelly in the grafts enhances the performance of allograft amniotic-derived, caderivic allograft, xenograft, or alloplast soft tissue substitutes.

Methods of making articles with a 3D printer using biomaterials that retain physical properties and biological activity are discussed. Methods can include providing a crosslinkable material and a biomaterial to a 3D printer, and crosslinking the materials to form an implant. Biomaterials can include, among other things, bone, or tissue.

The present invention provides a composition for kidney treatment using the omentum, a medical kit for kidney treatment including the composition for kidney treatment using the omentum, and a film for kidney treatment including a cured product of the composition for kidney treatment using the omentum.